AL 10 - Plant Transformation - Corn

Laboratory Activities - PlantStress Biotech INCT

• Integrate data from monocotyledon transcriptomes (rice, corn, wheat, Musa and Sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects.

• Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn).

• Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybean, cotton and corn).

• Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members.

• Generate GM soybean, GM cotton and GM corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance.

• Generate GM soybean, GM cotton and GM corn expressing sequences for silencing of insect genes and evaluate the phenotype obtained regarding the control of Helicoverpa armigera and Spodoptera frugiperda.

• Generate GM soybean, GM cotton and GM corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of H. armigera and S. frugiperda.

• Phenotyping (greenhouse or field) corn, soybean and cotton populations, resulting from crossing with GM events to obtain proof of concept of drought tolerance and/or resistance to S. frugiperda, H. armigera or Meloidogyne spp.