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AL 16 - Biotechnological Applications of Microorganisms Laboratory Description​ - PlantStress Biotech INCT Virus:plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. The main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. Research Lines Cell Biology, Biochemistry and Genetics of Microorganisms Biotechnological Applications of Microorganisms and their Products Microorganisms/Virus-Host Interactions Our Team Maite Vaslin de Freitas Silva Team Leader Associate Professor IV, member of PPG-Micro and PPG-PBV at UFRJ. Director of the Brazilian Society of Virology. Master's and PhD in Genetics from UFRJ in molecular biology of plants. Postdoc at IB UFRJ studying RNAi in antiviral defense. Visiting professor IB UFRJ 199-2001 and permanent professor at IMPG since 2002. I coordinate the Plant Molecular Virology Laboratory, whose lines are: the study of the virus: plant interaction and the identification of viruses associated with cotton diseases through sequencing and characterization of new viruses, development of molecular diagnostic tests, transcriptome and miRNA profile during viral infection. Today the main lines are the characterization of the molecular mechanism of resistance to CBD; the use of fungal molecules as inducers of protection against viral diseases; and the expression of SARS-CoV-2 antigens in plants. The group has 2 patents filed and 1 granted and is encouraging the birth of startups based on the research developed. Contact Maite Vaslin de Freitas Silva Federal University of Rio de Janeiro - UFRJ Department of Virology – Paulo de Góes Institute of Microbiology – UFRJ E-mail: maite@micro.ufrj.br Phone:+55 21 2560 8344

< Back Pesquisa do INCT PlantStress Biotech é destaque em encontro estudantil A pesquisa realizada no INCT PlantStress Biotech foi destaque durante o XXVII Encontro do Talento Estudantil, ocorrido na Embrapa Recursos Genéticos e Biotecnologia (Cenargen), em Brasília, entre 28 e 30 de agosto. Sara Vitorino da Rocha Lemes e Bruna Medeiros Pereira receberam a premiação de melhor trabalho na categoria Biotecnologia, em nível de graduação e pós-graduação, respectivamente. Sara Rocha é estudante de graduação em Biotecnologia na Universidade de Brasília (UnB) e bolsista de Iniciação Científica do programa PIBIC/CNPq/Embrapa, sob orientação das pesquisadoras Carolina Morgante e Fatima Grossi de Sá. Em seu trabalho, “Plantas de algodão geneticamente modificadas com redução da suscetibilidade a nematoides formadores de galhas e à seca”, Sara apresentou a estratégia combinada de superexpressão de um gene de tolerância à seca e de silenciamento de um gene vital do nematoide Meloidogyne incognita , usando a tecnologia do RNA interferente, para a obtenção de plantas menos suscetíveis a esses dois estresses, simultaneamente. Já Bruna Medeiros, mestre em Agronomia pela UnB e bolsista DTI-B/CNPq na Embrapa Cenargen, apresentou o trabalho “Uma abordagem trans-espécies para validação funcional in root e seleção de genes candidatos para resistência a fitopatógenos em leguminosas”. Sob orientação das pesquisadoras Ana Brasileiro e Patrícia Guimarães, Bruna destacou os resultados promissores de um sistema para rápida validação funcional de genes de resistência em raízes de soja, amendoim, grão de bico, feijão comum, feijão caupi, guandu e alfafa. Mais detalhes sobre esta pesquisa podem ser acessados nas recentes publicações nos periódicos PlosOne e Planta e na patente depositada pela equipe. As bolsistas Bruna Medeiros e Luanna Pinheiro recebendo a premiação de membros da comissão organizadora. Neste mesmo evento, receberam menção honrosa as estudantes Nayara Sabrina de Freitas Alves, doutoranda na Universidade Federal do Paraná, pelo trabalho “Modulação transcricional e traducional via CRISPR-Cas visando a tolerância da soja à nematoides de galhas” e Luanna Pinheiro A. F. Bezerra, doutoranda na Universidade Católica de Brasília, pelo trabalho “Plantas de soja tolerantes ao déficit hídrico por meio da modulação da via de controle de morte celular programada induzida por estresses utilizando CRISPR/dCas9”. As duas estudantes são orientadas pela pesquisadora Dra. Maria Fátima Grossi de Sá. O XXVII Encontro do Talento Estudantil contou com 150 participantes inscritos, entre estudantes de graduação, pós-graduação e pós-doutores. Teve como objetivos a divulgação dos trabalhos realizados na Embrapa Cenargen nas áreas temáticas de Biotecnologia; Controle Biológico, Quarentena e Recursos Genéticos e o incentivo científico a jovens estudantes e bolsistas. Texto: Dra. Carolina Morgante

Scholarship Opportunities Open opportunities 01 Opportunity Publication date: January 09th, 2024 Título da candidatura: Analista de pesquisa | Bolsista Transformação genética de soja Oportunidade de bolsa para área de Engenharia Genética. Procuramos profissionais criativos, com visão inovadora, com experiência em biotecnologia e biologia molecular. Responsibilidades Conduzir a transformação genética de soja para resistência a insetos; Caracterização molecular de eventos transgênicos, a nível de DNA, RNA e proetína; Realizar análises de expressão gênica (RNAm e proteína); Transformação de bactérias e células competentes; Elaboração e publicação de artigos e outros conteúdos sobre Biotecnologia e Biologia Molecular. Qualificação Graduação em Ciencias Biológicas, Agronomia ou Biotecnologia; Mestrado em biologia molecular, biologia celular, fisiologia, biotecnologia ou áreas afins; Experiência em transformação genética de plantas, caracterização molecular, cultura de tecidos, RTqPCR; Domínio da língua inglesa (leitura e escrita); Disponibilidade para trabalho presencial 40 horas semanais em Brasília, DF; Boas habilidades de escrita; Conhecimento no pacote Microsoft Office; Disposto a aprender sobre novas tecnologias; Criatividade e proatividade. Período: 12 meses Início: Março de 2024 Valor da bolsa: R$ 4.000,00 Local: Laboratório de Interação Molecular Planta-Praga | EMBRAPA Recursos Genéticos e Biotecnologia, Brasília (DF) Prazo de submissão: 30 de janeiro de 2024 Documentos necessários: Carta de motivação (descrevendo principais competências, experiências e objetivos) e link para o CV Lattes atualizado. Enviar candidatura para: inctplantstressbiotech@gmail.com Assunto: Bolsa Transformação soja 02 Opportunity Publication date: January 18th, 2024 Título do trabalho: Bolsa de Pós Doutorado visando apoiar projeto de Desenvolvimento de Diagnósticos Moleculares e Sorológicos do Tipo Point of Care. Responsabilidades: Executar atividades de desenvolvimento e produção de anticorpos Policlonais e Monoclonais, ensaios de ELISA, RT-PCR, qPCR (PCR em tempo Real), Desenvolver teste do Tipo Point of Care; além da produção de conteúdos técnico-científicos. Requisitos: Doutorado em Biologia Molecular, Bioquímica, Biologia Celular, Imunologia e áreas afins. Experiência requerida: Biologia molecular e Biotecnologia Período : 24 meses Valor da bolsa: R$ 6.900,00 Local: EMBRAPA Recursos Genéticos e Biotecnologia, Brasília (DF) Prazo de submissão: Até 09 de fevereiro de 2024 Documentos necessários: Curriculo Lattes atualizado nos últmos 2 meses. Diploma de Doutorado e Histórico Escolar do Doutourado. Contato para candidatura: emanuel.abreu@embrapa.br 03 Opportunity Publication date: June 14th, 2022 Título do trabalho: Transformação de soja Responsabilidades: Transformação genética de plantas, cultura de tecidos vegetais, análises moleculares Requisitos: Mestrado em biologia molecular, biologia celular, fisiologia, biotecnologia ou áreas afins. Período : 12 meses Valor da bolsa: R$ 3.000,00 Local: LA 9 - EMBRAPA Recursos Genéticos e Biotecnologia, Brasília (DF) Prazo de submissão: 30 de junho de 2022. Documentos necessários: Carta de motivação e link para o CV Lattes atualizado. Contato para candidatura: carolina.morgante@embrapa.br 04 Opportunity Publication date: March 11 th, 2022 Título do trabalho: Cultivo de raízes do tipo "hairy roots" de espécies silvestres de Arachis Responsabilidades: Transformação genética de plantas, cultura de tecidos vegetais, análises moleculares Requisitos: Doutorado em biologia molecular, biologia celular, fisiologia, biotecnologia ou áreas afins Período: 2022-2023, renovável por mais 1 ano Valor da bolsa: R$ 4.300,00 Local: LA 5 - EMBRAPA Recursos Genéticos e Biotecnologia, Brasília (DF) Contato para candidatura: ana.brasileiro@embrapa.br 05 Opportunity Publication date: March 11 th, 2022 Título do trabalho: Edição de genes de suscetibilidade (S) e de resistência (R) em plantas compostas de soja Responsabilidades: Edição de genes (CRISPR), análises moleculares, análise de bioinformática Requisitos: Doutorado em biologia molecular, bioinformática, biologia celular Período: 2022-2023, renovável por mais 1 ano Valor da bolsa: R$ 4.300,00 Local: LA 5 - EMBRAPA Recursos Genéticos e Biotecnologia , Brasília (DF) Contato para candidatura: patricia.guimaraes@embrapa.br

Publications Scientific Papers RELEVANT SCIENTIFIC PAPERS (FROM 2017) 2021 ​ GUTERRES JM, Rocha AJ, Nascimento FS, Santos AS, Miller RNG, Ferreira CF, Haddad F, Amorim VB (2021) . Genetic improvement for resistance to Black Sigatoka in bananas: a systematic review. Frontiers in Plant Science , 12, 657916. https://doi.org/10.3389/fpls.2021.657916 ​ ALBUQUERQUE GMR, Fonseca FCA, Boiteux LS, Borges RC, Miller RNG, Lopes CA, Souza EB, Fonseca MEN (2021) . Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum -tomato ‘Hawaii 7996’ interactions. Scientific Reports , 11, 1. https://doi.org/10.1038/s41598-021-97854-8 ​ CAPELARI ÉF, Anjos L, Rodrigues NF, Sousa RMJ, Silvera JAG, Margis R (2021) . Transcriptional profiling and physiological responses reveal new insights into drought tolerance in a semiarid adapted species, Anacardium occidentale . Plant Biology , 24, 1-12. https://doi.org/10.1111/plb.13312 ​ ERLANDSON SR, Margis R, Ramirez A, Nguyen N, Lofgren L, Liao HL, Vilgalys R, Kennedy PG, Peay KG (2021) . Transcriptional acclimation and spatial differentiation characterize drought response by the ectomycorrhizal fungus Suillus pungens. New Phytologist , 234, 1-4. https://doi.org/10.1111/nph.17816 ​ MENDES-BEZERRA AC, Valença DC, Junqueira NEG, Hüther CM, Borella J, Ferreira-de-Pinho C, Ferreira MA, Medici LO, Ortiz-Silva B, Reinert F (2021) . Potassium supply promotes the mitigation of NaCl-induced effects on leaf photochemistry, metabolism and morphology of Setaria viridis . Plant Physiology and Biochemistry , 160, 1. https://doi.org/10.1016/j.plaphy.2021.01.021 ​ CABRAL LM, Masuda HP, Ballesteros HF, Almeida-Engler J, Alves-Ferreira M, Toni KLG, Bizotto FM, Ferreira PCG, Hemerly AS (2021) . ABAP1 plays a role in the differentiation of male and female gametes in Arabidopsis thaliana . Frontiers in Plant Science , 12, 642758. https://doi.org/10.3389/fpls.2021.642758 ​ BASSO MF, Costa JA, Ribeiro TP, Arraes FBM, Lourenço-Tessutti IT, Macedo AF, Neves MR, Nardeli SM, Arge LW, Perez CEA, Silva PLR, Macedo LLP, Lisei-De-Sa ME, Santos-Amorim RM, Pinto ERC, Silva MCM, Morgante CV, Floh EIS, Alves-Ferreira M, Grossi-De-Sa MF (2021) . Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum ) improves drought tolerance. Plant Physiology and Biochemistry, 165, 80-93. https://doi.org/10.1016/j.plaphy.2021.05.009 ​ TRAVASSOS-LINS J, Oliveira-Rocha CC, Souza-Rodrigues T, Alves-Ferreira M (2021) . Evaluation of the molecular and physiological response to dehydration of two accessions of the model plant Setaria viridis . Plant Physiology and Biochemistry, 169, 43. https://doi.org/10.1016/j.plaphy.2021.11.015 ​ VALERIANO FR, Moura SM, Travassos-Lins J, Alves-Ferreira M, Vieira RC, Ortiz-Silva B, Reinert F (2021) . Evaluation of Setaria viridis responses to salt treatment and potassium supply: a characterization of three contrasting accessions. Brazilian Journal of Botany , 44, 1. https://doi.org/10.1007/s40415-021-00773-1 ​ NETO MCL, Carvalho FEL, Souza GM, Silveira JAG (2021) . Understanding photosynthesis in a spatial-temporal multiscale: The need for a systemic view. Theoretical and Experimental Plant Physiology , 33, 113-124. https://doi.org/10.1007/s40626-021-00199-w ​ SOUSA RT, Paiva ALS, Carvalho FEL, Alencar VTCB, Silveira JAG (2021) . Ammonium overaccumulation in senescent leaves as a novel exclusion mechanism to avoid toxicity in photosynthetically active rice leaves. Environmental and Experimental Botany , 186, 104452. https://doi.org/10.1016/j.envexpbot.2021.104452 ​ ARAUJO ACG, Guimaraes PM, Guimaraes LA, Martins ACQ, Mota APZ, Pereira BM, Brasileiro ACM (2021) . Overexpression of DUF538 from wild Arachis enhances plant resistance to Meloidogyne spp . Agronomy-Basel , 11, 559. https://doi.org/10.3390/agronomy11030559 ​ MOTA APZ, Brasileiro ACM, Vidigal B, Oliveira TN, Martins ACQ, Saraiva MAP, Araujo ACG, Togawa RC, Grossi-de-Sa MF, Guimaraes PM (2021) . Defining the combined stress response in wild Arachis . Scientific Reports, 11, 1-16. https://doi.org/10.1038/s41598-021-90607-7 ​ BRASILEIRO ACM, Lacorte C, Pereira BM, Oliveira TN, Ferreira DS, Mota APZ, Saraiva MAP, Araujo ACG, Silva LP, Guimaraes PM (2021) . Ectopic expression of an gene from wild Arachis enhances tolerance to both abiotic and biotic stresses. Plant Journal , 1, tpj.15409. https://doi.org/10.1111/tpj.15409 ​ OLIVEIRA-BUSATTO LA, Almeida RMC, Weber RLM, Favero D, Bredemeier C, Silva-Giordano CP, Bodanese-Zanettini MH (2021) . The soybean transcriptogram allows a wide genome-to-single-gene analysis that evinces time-dependent drought response. Plant Molecular Biology Reporter , 12, 1. https://doi.org/10.1007/s11105-021-01297-4 ​ FAILLACE GR, Caruso PB, Timmers LFS, Favero D, Guzman FL, Rechenmacher C, Oliveira-Busatto LA, Souza ON, Bredemeier C, Bodanese-Zanettini MH (2021) . Molecular characterisation of soybean osmotins and their involvement in drought stress response. Frontiers in Genetics , 12, 1. https://doi.org/10.3389/fgene.2021.632685 ​ RODRIGUES-SILVA PL, Fernandes PBB, Rodrigues MT, Figueiredo LHM, Grossi-de-Sa MF (2021) . Tendências quanto ao conhecimento e às aplicações biotecnológicas do Psidium guineense evidenciadas pelo monitoramento tecnológico. Cadernos de Ciência & Tecnologia , 38, e26704. http://dx.doi.org/10.35977/0104-1096.cct2021.v38.26704 ​ MENDES RAG, Basso MF, Paes-de-Melo B, Ribeiro TP, Lima RN, Araujo JF, Grossi-de-Sa M, Mattos VS, Togawa RC, Albuquerque EVS, Lisei-de-Sá ME, Silva MCM, Macedo LLP, Fragoso RR, Fernandez D, Vignols F, Grossi-de-Sa MF (2021) . The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , 114, 101630. https://doi.org/10.1016/j.pmpp.2021.101630 ​ CABRAL D, Forero-Ballesteros HF, Paes-de-Melo B, Lourenço-Tessutti IT, Siqueira KMS, Obicci L, Grossi-de-Sa MF, Hemerly AS, Almeida-Engler J (2021) . The armadillo BTB protein ABAP1 is a crucial player in DNA replication and transcription of nematode-induced galls. Frontiers in Plant Science , 12, 636663. https://doi.org/10.3389/fpls.2021.636663 ​ MOREIRA-PINTO CE, Coelho RR, Leite AGB, Silveira DA, Souza DA, Lopes RB, Macedo LLP, Silva MCM, Ribeiro TP, Antonino JD, Grossi-de-Sa MF (2021) . Increasing susceptibility to Anthonomus grandis through RNAi-induced AgraRelish knockdown: a perspective to combine biocontrol and biotechnology. Pest Management Science , 77, 4054-4063. https://doi.org/10.1002/ps.6430 . ​ BASSO MF, Costa JA, Ribeiro TP, Arraes FBM, Lourenço-Tessutti IT, Macedo AF, Neves MRS, Nardeli SM, Arge LW, Perez CEA, Silva PLR, Macedo LLP, Lisei-de-Sá ME, Amorim RMS, Pinto ERC, Silva MCM, Morgante CV, Floh EIS, Alves-Ferreira M, Grossi-de-Sa MF (2021) . Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum ) improves drought tolerance. Plant Physiology and Biochemistry , 165, 80-93. https://doi.org/10.1016/j.plaphy.2021.05.009 . ​ PAES-DE-MELO B, Lourenço-Tessutti IT, Fraga OT, Pinheiro LB, Jesus-Lins CB, Morgante CV, Almeida-Engler J, Reis PAB, Grossi-de-Sa MF, Fontes EPB (2021) . Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses, and cell death responses. Scientific Reports , 11, 11178. https://doi.org/10.1038/s41598-021-90767-6 . ​ MOTA APZ, Brasileiro ACM, Vidigal B, Oliveira TN, Martins ACQ, Saraiva MAP, Araújo ACG, Togawa RC, Grossi-de-Sa MF, Guimaraes PM (2021) . Defining the combined stress response in wild Arachis . Scientific Reports , 11, 11097. https://doi.org/10.1038/s41598-021-90607-7 . ​ RIBEIRO TP, Lourenço-Tessutti IT, Paes-De-Melo B, Morgante CV, Filho AS, Lins CBJ, Ferreira GF, Mello GN, Macedo LLP, Lucena WA, Silva MCM, Oliveira-Neto OB, Grossi-de-Sa MF (2021) . Improved cotton transformation protocol mediated by Agrobacterium and biolistic combined-methods. Planta , 254, 20. https://doi.org/10.1007/s00425-021-03666-5 . ​ ARAUJO-SOUSA B, Nascimento-Silva O, Farias-Porto W, Lima-Rocha T, Paulino-Silva L, Ferreira-Leal AP, Buccini DF, Fajemiroye JO, Araújo-Caldas R, Franco OL, Grossi-de-Sa MF, Fuente-Nunez C, Moreno SE (2021) . Identification of the active principle conferring anti-inflammatory and antinociceptive properties in bamboo plant. Molecules , 26, 3054. HTTPS://DOI.ORG/10.3390/MOLECULES26103054 . ​ GODINHO-MENDES RA, Basso MF, Fernandes-de-Araújo J, Paes-de-Melo B, Lima RN, Ribeiro TP, Silva-Mattos V, Saliba-Albuquerque EV, Grossi-de-Sa M, Dessaune-Tameirao SN, Fragoso RR, Mattar-da-Silva MC, Vignols F, Fernandez D, Grossi-de-Sa MF (2021) . Minc00344 and Mj-NULG1a effectors interact with GmHub10 protein to promote the soybean parasitism by Meloidogyne incognita and M. javanica . Experimental Parasitology , 229, 108153. https://doi.org/10.1016/j.exppara.2021.108153 . ​ LISEI-DE-SÁ ME, Rodrigues-Silva PL, Morgante CV, Paes-de-Melo B, Lourenço-Tessutti IT, Arraes FBM, Sousa JPA, Galbieri R, Amorim RMS, Lins CBJ, Macedo LLP, Moreira VJ, Ferreira GF, Ribeiro TP, Fragoso RR, Silva MCM, Almeida-Engler J, Grossi-de-Sa MF (2021) . Pyramiding dsRNAs increases phytonematode tolerance in cotton plants. Planta , 254, 121. https://doi.org/10.1007/s00425-021-03776-0 . ​ PAES-DE-MELO B, Moura SM, Morgante CV, Pinheiro DH, Alves NSF, Rodrigues-Silva PL, Lourenço-Tessutti IT, Andrade RV, Fragoso RR, Grossi-de-Sa MF (2021) . Regulated promoters applied to plant engineering: an insight over promising soybean promoters under biotic stress and their cis-elements. Biotechnology Research and Innovation , 5, e2021005. https://doi.org/10.4322/biori.202105 . ​ ARRAES FBM, Martins-de-Sa D, Vasquez DDN, Melo BP, Faheem M, Macedo LLP, Morgante CV, Barbosa JARG, Togawa RC, Moreira VJV, Danchin EGJ, Grossi-de-Sa MF (2021) . Dissecting protein domain variability in the core RNA interference machinery of five insect orders. RNA Biology , 18, 1653-1681. https://doi.org/10.1080/15476286.2020.1861816 . ​ GODINHO-MENDES RA, Basso MF, Melo BP, Ribeiro TP, Lima RN, Fernandes-de-Araújo J, Grossi-de-Sa M, Silva-Mattos V, Togawa RC, Saliba-Albuquerque ÉV, Lisei-de-Sa ME, Mattar-da-Silva MC, Macedo LLP, Fragoso RR, Fernandez D, Vignols F, Grossi-de-Sa MF (2021) . The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita . Physiological and Molecular Plant Pathology , 114, 101630. https://doi.org/10.1016/j.pmpp.2021.101630 . ​ MELLO FE, Lopes-Caitar VS, Prudente H, Xavier-Valencio SA, Franzenburg S, Mehl A, Marcelino-Guimaraes FC, Verreet JA, Balbi-Peña MI, Godoy CV (2021) . Sensitivity of Cercospora spp . from soybean to quinone outside inhibitors and methyl benzimidazole carbamate fungicides in Brazil. Tropical Plant Pathology , 1. https://doi.org/10.1007/s40858-020-00410-4 . ​ ALEKCEVETCH JC, Lima-Passianotto AL, Ferreira EG, Santos AB, Silva DCG, Dias WP, Belzile F, Abdelnoor RV, Marcelino-Guimaraes FC (2021) . Genome-wide association study for resistance to Meloidogyne javanica causing root-knot nematode in soybean. Theoretical and Applied Genetics , 134, 777-792. https://doi.org/10.1007/s00122-020-03723-9 . ​ SILVA E, Perez-da-Graça J, Porto C, Prado RM, Nunes E, Marcelino-Guimarães FC, Meyer MC, Jorge-Pilau E (2021). Untargeted metabolomics analysis by UHPLC-MS/MS of soybean plant in a compatible response to Phakopsora pachyrhizi infection. Metabolites , 11, 179. https://doi.org/10.3390/metabo11030179 . ​ SANTOS IMO, Abe VY, Carvalho K, Barazetti AR, Simionato AS, Almeida-Pega GE, Matis SH, Cano BG, Cely MV, Marcelino-Guimaraes FC, Chryssafidis AL, Andrade G (2021) . Secondary metabolites of Pseudomonas aeruginosa LV strain decrease Asian soybean rust severity in experimentally infected plants. Plants , 10, 1495. https://doi.org/10.3390/plants10081495 . ​ MELLO FE, Lopes-Caitar VS, Xavier-Valencio SA, Silva HP, Franzenburg S, Mehl A, Verreet JA, Balbi-Peña MI, Marcelino-Guimaraes FC, Godoy CV (2021) . Resistance of Corynespora cassiicola from soybean to QoI and MBC fungicides in Brazil. Plant Pathology (Online), 71, 1-16. https://doi.org/10.1111/ppa.13474 . ​ FREITAS KJ, Santos RS, Busanello C, Victoria FC, Lopes JL, Wing R, Oliveira AC (2021) . Starch synthesis-related genes (SSRG) evolution in the genus Oryza . Plants , 10, 1-15. https://doi.org/10.3390/plants10061057 . ​ VIANA VE, Maia LC, Busanello C, Pegoraro C, Oliveira AC (2021) . When rice gets the chills: comparative transcriptome profiling at germination shows WRKY transcription factor responses. Plant Biology , 23, 100-112. https://doi.org/10.1111/plb.13262 . ​ MAGALHÃES-DA-FONSECA G, Nardino M, Luz VK, Oliveira VF, Magalhães-da-Fonseca M, Magalhães-Bandeira J, Magalhães-Júnior AM, Maia LC, Oliveira AC (2021) . Gene introgression for imidazolinone group chemical herbicide tolerance. Cereal Research Communications , 49, 457-463. https://doi.org/10.1007/s42976-020-00126-w . ​ OLIVEIRA VF, Busanello C, Viana VE, Stafen CF, Pedrolo AM, Paniz FP, Pedron T, Pereira RM, Rosa SA, Magalhães-Júnior AM, Oliveira AC, Batista BL, Pegoraro C (2021) . Assessing mineral and toxic elements content in rice grains grown in southern Brazil. Journal of Food Composition and Analysis , 100, 103914. https://doi.org/10.1016/j.jfca.2021.103914 . ​ NIZOLLI VO, Pegoraro C, Oliveira AC (2021) . Rice blast: strategies and challenges for improving genetic resistance. Crop Breeding and Applied Biotechnology , 21, e387721S9. https://doi.org/10.1590/1984-70332021v21Sa22 . ​ OLIVEIRA-MAXIMINO JV, Barros LM, Pereira RM, Santi II, Aranha BC, Busanello C, Viana VE, Freitag RA, Batista BL, Oliveira AC, Pegoraro C (2021) . Mineral and fatty acid content variation in white oat genotypes grown in Brazil. Biological Trace Element Research , 199, 1194-1206. https://doi.org/10.1007/s12011-020-02229-1 . ​ MALTZAHN LE, Zenker SG, Lopes JL, Pereira RM, Verdi CA, Rother V, Busanello C, Viana VE, Batista BL, Oliveira AC, Pegoraro C (2021) . Brazilian genetic diversity for desirable and undesirable elements in the wheat grain. Biological Trace Element Research , 199, 2351-2365. https://doi.org/10.1007/s12011-020-02338-x . ​ VIANA VE, Maltzahn LE, Oliveira AC, Pegoraro C (2021) . Genetic approaches for iron and zinc biofortification and arsenic decrease in Oryza sativa L. grains. Biological Trace Element Research , 10, 4505-4523. https://doi.org/10.1007/s12011-021-03018-0 . ​ FERNANDES RC, Busanello C, Viana VE, Venske E, Oliveira VF, Lopes JL, Maia LC, Oliveira AC, Pegoraro C (2021) . Genetic variability and heritability of agronomic traits in a wheat collection used in southern Brazil. Journal of Crop Science and Biotechnology (Seoul), 25, 337-348. https://doi.org/10.1007/s12892-021-00135-z . ​ ERLANDSON SR, Margis R, Ramirez A, Nguyen N, Lofgren LA, Liao H, Vilgalys R, Kennedy PG, Peay KG (2021) . Transcriptional acclimation and spatial differentiation characterize drought response by the ectomycorrhizal fungus Suillus pungens . New Phytologist . https://doi.org/10.1111/nph.17816 . ​ GALBIERI R, Kobayasti L, Albuquerque MCF, Sá RO, Dutra SG, Boldt AS, Timper P (2021) . Castor bean as an option for management in cotton. 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Multi-effect of the water-soluble Moringa oleifera lectin against Serratia marcescens and Bacillus sp.: antibacterial, antibiofilm and anti-adhesive properties. Journal of Applied Microbiology , 123:861. https://doi.org/10.1111/jam.13556 SOUZA RAV, Alves MDC, Carneiro NP, Barros BDA, Borém A, Carneiro AA (2017) . Agrobacterium-mediated genetic transformation of a tropical elite maize line. Crop Breeding and Applied Biotechnology , 17:133-140. https://doi.org/10.1590/1984-70332017v17n2a20 RAMOS-COELHO R, Vieira P, Antonino-de-Souza-Júnior JD, Martin-Jimenez C, Veylder L, Cazareth J, Engler G, Grossi-de-Sa MF, Almeida-Engler J (2017) . Exploiting cell cycle inhibitor genes of the KRP family to control root-knot nematode induced feeding sites in plants. Plant, Cell and Environment (Print), 40:27. https://doi.org/10.1111/pce.12912 MOURA SM, Artico S, Lima C, Nardeli SM, Berbel A, Oliveira-Neto OB, Grossi-de-Sa MF, Ferrándiz C, Madueño F, Alves-Ferreira M (2017) . Functional characterization of AGAMOUS-subfamily members from cotton during reproductive development and in response to plant hormones. Plant Reproduction , 29:1-21. https://doi.org/10.1007/s00497-017-0297-y HABIBI P, Prado GS, Pelegrini PB, Hefferon KL, Soccol CR, Grossi-de-Sá MF (2017) . Optimization of inside and outside factors to improve recombinant protein yield in plant. Plant Cell Tissue and Organ Culture , 129:1-19. https://doi.org/10.1007/s11240-017-1240-5 GILLET FX, Bournaud C, Souza-Jr JDA, Grossi-de-Sa MF (2017) . Plant-parasitic nematodes: towards understanding molecular players in stress responses. Annals of Botany , v. 119, p. 775-789. https://doi.org/10.1093/aob/mcw260 GILLET FX, Garcia RA, Macedo LLP, Albuquerque EVS, Silva MCM, Grossi-de-Sa MF (2017) . Investigating Engineered Ribonucleoprotein Particles to Improve Oral RNAi Delivery in Crop Insect Pests. 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Agricultural Sciences , 08:1195-1225. https://doi.org/10.4236/as.2017.810087 ALMEIDA-GARCIA R, Macedo LLP, Cabral-do-Nascimento D, Gillet FX, Moreira-Pinto CE, Faheem M, Moreschi-Basso AM, Mattar-Silva MC, Grossi-de-Sa MF (2017) . Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis . PLoS One , 12. https://doi.org/10.1371/journal.pone.0189600 FAUSTO AKS, Silva TDF, Romanell E, Vaslin MFS (2017) . MicroRNAs as reference genes for quantitative PCR in cotton. PLoS One , 12. https://doi.org/10.1371/journal.pone.0174722 GALBIERI R, Boldt A, Scoz LB, Rodrigues S, Rabel DO, Belot JL, Vaslin MFS, Kobayasti L, Chitarra L (2017) . Cotton Blue Disease in Brazil: occurrence, vector (Aphis gossypii ) control level and cultivars reaction. Tropical Plant Pathology , 43:1-7. https://doi.org/10.1007/s40858-017-0165-1 DOMITROVIC T, Fausto AK, Silva TDF, Romanell E, Vaslin MFS (2017) . Plant arginyltransferases (ATEs). Genetics and Molecular Biology (Online Version), 40:253-260. https://doi.org/10.1590/1678-4685-GMB-2016-0084 FUSARO A, Barton D, Nakasugi K, Jackson C, Kalischuk M, Kawchuk L, Vaslin M, Correa R, Waterhouse P (2017) . The Luteovirus P4 Movement Protein Is a Suppressor of Systemic RNA Silencing. Viruses-Basel , 9:294. https://doi.org/10.3390/v9100294 MEYER MC, Favoreto L, Klepker D, Marcelino-Guimarães FC (2017) . Soybean green stem and foliar retention syndrome caused by Aphelenchoides besseyi . Tropical Plant Pathology , 42:403-409. https://doi.org/10.1007/s40858-017-0167-z PASSIANOTTO ALL, Sonah H, Dias WP, Marcelino-Guimarães FC, Belzile F, Abdelnoor RV (2017) . Genome-wide association study for resistance to the southern root-knot nematode (Meloidogyne incognita ) in soybean. Molecular Breeding , 37:148. https://doi.org/10.1007/s11032-017-0744-3 LUCHE HS, Silva JAG, Nornberg R, Hawerroth MC, Silveira SFS, Caetano VDR, Santos RL, Figueiredo RG, Maia LC, Oliveira AC (2017) . Stay-green character and its contribution in Brazilian wheats. Ciência Rural , 47. https://doi.org/10.1590/0103-8478cr20160583 SANTOS RS, Farias DR, Pegoraro C, Rombaldi CV, Fukao T, Wing RA, Oliveira AC (2017) . Evolutionary analysis of the SUB1 locus across the Oryza genomes. Rice , 10:4. https://doi.org/10.1186/s12284-016-0140-3 SANTOS RS, Araujo-Junior AT, Pegoraro C, Costa-de-Oliveira A (2017) . Dealing with iron metabolism in rice: from breeding for stress tolerance to biofortification. Genetics and Molecular Biology , 40:312-325. https://doi.org/10.1590/1678-4685-GMB-2016-0036 BARETTA D, Nardino M, Carvalho IR, Pelegrin AJ, Ferrari M, Szareski V, Barros WS, Souza VQ, Costa-de-Oliveira A, Maia LC (2017) . Estimates of genetic parameters and genotypic values prediction in maize landrace populations by REML/BLUP procedure. Genetics and Molecular Research , 16:1. http://dx.doi.org/10.4238/gmr16029715 LUCHE HS, Silva JAG, Nornberg R, Arenhardt EG, Rosa-Caetano V, Maia LC, Costa-de-Oliveira A (2017) . Prediction of parental combination for introduction of stay-green associated loci in wheat. Journal of Crop Science and Biotechnology (Seoul), 20:73-80. https://doi.org/10.1007/s12892-016-0127-0 ARENHARDT EG, Silva JAG, Arenhardt LG, Silva DR, Gzergorczick ME, Ceolin GP, Stülp C, Figueiredo RG, Oliveira AC (2017) . Technical and agronomic efficiency of oat cultivars as a function of nitrogen availability. Científica (Jabotical), 45:257-270. https://doi.org/10.15361/1984-5529.2017v45n3p257-270 VIANA VE, Marini N, Finatto T, Ezquer I, Busanello C, Santos R, Pegoraro C, Colombo L, Costa-de-Oliveira A (2017) . Iron excess in rice: from phenotypic changes to functional genomics of WRKY transcription factors. Genetics and Molecular Research , 16:1. https://dx.doi.org/10.4238/gmr16039694 ​ ​ ​

< Back CAPES Thesis Award 2021 Tese premiada pela Capes valida sistema de melhoramento por edição de genoma e apresenta novo protocolo para transformação da soja O interesse pela genética de plantas sempre conduziu a vida acadêmica do bioquímico Bruno Paes de Melo, que se dedicou a ela na graduação na UFV, como bolsista de iniciação científica, no mestrado e no doutorado, esses realizados no Programa de Pós-Graduação em Bioquímica Aplicada . Foi em sua última experiência como estudante da UFV que Bruno desenvolveu a tese Transcriptional modulation and characterization of plant-specific transacting factors , defendida em 2020. O trabalho rendeu a ele destaque na 16ª edição do Prêmio Capes de Tese, cujo resultado foi divulgado no dia 3 de setembro. Orientada pela professora Elizabeth Pacheco Batista Fontes – sua orientadora desde a iniciação científica -, a tese ficou entre as 49 selecionadas das 1.376 avaliadas de todo o país. Na pesquisa premiada pela Capes na área de Ciências Agrárias I, Bruno explorou a funcionalidade de alguns fatores de transcrição (reguladores centrais da expressão gênica nas células) em plantas submetidas a diferentes situações de estresse. Sua exploração acabou revelando à comunidade científica novos alvos e, consequentemente, novas metodologias para o melhoramento genético moderno ou biotecnológico. Com seu estudo, o pesquisador apresentou maneiras inovadoras de se fazer plantas com performances melhores diante de diferentes desafios. A tese tem quatro capítulos que Bruno define como “diferentes entre si, mas com dois focos”: a validação de um sistema de melhoramento biotecnológico por edição de genoma e a otimização de um protocolo para transformação genética da soja e a caracterização de novos genes-alvo para esse fim. No que diz respeito à validação do sistema de melhoramento, a proposta do Bruno foi explorar a funcionalidade de AREB-1, um fator de transcrição, da planta do gênero Arabidopsis , que descende de um ancestral comum de algumas hortaliças, como a couve e a mostarda. A opção em explorar a Arabidopsis se deu pelo fato de que, no universo das plantas, ela é considerada modelo, já que tem todo um genoma muito bem descrito e as vias metabólicas e de sinalização celular bem elucidadas. “É uma planta em que a transformação genética é muito fácil”, explica Bruno. Segundo ele, quando se faz um trabalho em Arabidopsis , sabe-se o que esperar. “Como eu precisava testar uma nova estratégia, isso precisaria ser feito num sistema que eu conhecesse a resposta, para saber se havia ou não dado certo”. Bruno descreveu uma nova estratégia de modulação da transcrição de AREB-1 por CRISPR/dCas9 em Arabidopsis para tolerância à seca. O AREB-1 é extensivamente caracterizado nas adaptações fisiológicas ao estresse hídrico. Ou seja, a função desse gene é conferir à planta maior tolerância ao estresse hídrico. Assim, plantas que têm a expressão deste gene aumentada são mais tolerantes à seca. Em sua pesquisa, Bruno aumentou a transcrição deste gene utilizando CRISPR/dCas9, uma estratégia de modulação transcricional baseada em edição de genoma que ainda é muito nova, tendo despontado na biotecnologia há menos de 10 anos. Ele fez isso a partir de uma alteração na cromatina (complexo de DNA e proteínas que se encontra dentro do núcleo celular) de modo a facilitar o acesso da maquinaria de transcrição ao local onde o gene AREB-1 se encontra. Essa abordagem usada para modular a expressão de um gene foi inovadora. O pesquisador explica que, geralmente, quando se quer fazer a modulação de um gene ou se coloca um promotor de vírus, que fica expressando todo o tempo na planta, ou se faz um silenciamento para inibir aquele gene. “O que fiz foi uma alteração do genoma em nível estrutural. Eu alterei a forma do genoma e isso fez com que a expressão desse gene aumentasse”, conta o pesquisador. Por isso, justifica, “escolhi a Arabidopsis , porque se a expressão do gene aumentasse, eu já sabia tudo o que iria acontecer com a planta”. Segundo Bruno, a superexpressão do gene AREB-1 mediada por CRISPR promoveu uma melhora no desempenho fisiológico das plantas transgênicas em 30 dias de privação de água. Os resultados revelam, portanto, uma estratégia molecular que permite a ativação racional de genes endógenos em plantas por meio de modulação da atividade da cromatina direcionada a um interesse agronômico. “Com essa edição do genoma, eu consegui obter plantas que, mesmo em déficit severo de água, tiveram boa performance, ou seja, não morreram e permaneceram verdes e produtivas. Eu validei uma estratégia que pode ser aplicada em grandes e quaisquer culturas, como a soja. A estratégia é universal”. Funções da família NAC na soja Durante o doutorado, Bruno também aprofundou seus estudos em fatores de transcrição NAC, genes que ele pesquisa desde 2013, que foi, inclusive, objeto de sua dissertação de mestrado. NAC é uma superfamília com 180 membros de genes, número atualizado em sua pesquisa de mestrado. Até então, apenas 132 genes eram descritos como pertencentes à família NAC na soja. Na pesquisa premiada pela Capes, o objetivo foi explorar as funções de dois genes NAC no controle de respostas a estresses e à senescência na soja. A proposta era elencar possíveis alvos para o melhoramento molecular, pois a partir do momento em que se conhece o gene e a função dele, se sabe como manipulá-lo dentro da planta com as características que se deseja. Bruno conta que os fatores de transcrição da família NAC têm o que se chama de plasticidade funcional: “alguns deles conferem tolerância a estresses múltiplos”. Tal característica permitiu que o pesquisador explorasse genes que tinham papéis contrastantes: um deles conferia resistência ou tolerância a vários tipos de estresse e atenuava a senescência e outro fazia justamente o contrário, aumentava a suscetibilidade da planta a estes mesmos estresses e acelerava a senescência. Bruno fez a transformação de Arabidopsis com estes genes para conferir se o efeito sobre a planta era o que se desejava para uma característica agronômica, visando transformar a soja. “Como a transformação da soja é difícil, o que se constitui num aspecto limitante para o seu melhoramento, eu desenvolvi um protocolo para facilitar a transformação desta planta e melhorar a eficiência deste processo”, conta o pesquisador. Para isso, ele combinou duas técnicas na metodologia para a transformação genética da soja: a biolística (transferência direta de DNA em uma célula para criação de organismos transgênicos) e a transformação mediada por Agrobacterium tumefaciens (bactéria do solo bastante utilizada na geração de plantas transgênicas). Na prática, Bruno fez microferidas em células do eixo embrionário da soja aumentando a infectividade da bactéria, que é capaz de transferir um DNA exógeno para a planta. Em geral, os protocolos atuais que empregam a Agrobacterium tumefaciens ou a biolística exibem baixa eficiência e exigem etapas sucessivas de cultivo e regeneração de plantas in vitro , com extensas perdas por contaminação e escurecimento do tecido. No protocolo desenvolvido por Bruno, a soja é transformada e regenerada in vitro em um único passo, reduzindo, assim, o tempo de geração das plantas transgênicas. De acordo com o pesquisador, num melhoramento convencional, este tempo pode chegar a até 12 meses. Com o seu protocolo, o processo de regeneração da soja é finalizado em até seis semanas. Além disso, a alta capacidade regenerativa do eixo embrionário permite alongamento do caule, desenvolvimento radicular e regeneração da planta. Durante a sua investigação, Bruno também identificou 32 novos genes NAC putativos, ou seja, genes que, apesar de terem as mesmas características dos NAC já descritos, não podem ser assim considerados plenamente pelo fato de que nem todos foram validados. Com essa descoberta, o pesquisador atualizou a superfamília no genoma da soja que já tinha 180 membros. “Já era uma família bem descrita, com muitos membros já caracterizados. Com a descoberta de 32 novos genes-alvo, abrem-se mais possibilidades para o melhoramento biotecnológico explorar suas diferentes funções na resistência a estresses específicos”. Importância O pesquisador que atua numa multinacional do mercado de sementes lembra que, atualmente, grande parte das cultivares produzidas no Brasil é transgênica. Em sua opinião, os transgênicos vieram para, dentre outras possibilidades, melhorar a produção, a resistência à praga e o desempenho das plantas, especialmente em momentos como o que estamos vivendo de grandes mudanças climáticas. Por essa razão, considera que seu trabalho traz uma importante contribuição à agricultura. “Estou mostrando novos métodos de se fazer transgênicos e um melhoramento muito mais associado à biotecnologia do que ao melhoramento clássico”. Nesse último, de acordo com Bruno, se cruza, por exemplo, uma planta resistente com uma outra que produz muito para se obter uma planta resistente e produtiva. “Agora, com o melhoramento biotecnológico, se consegue colocar as duas características ao mesmo tempo na planta”. A pesquisa de Bruno teve o apoio da Capes e foi realizada no Laboratório de Biologia Molecular de Plantas do Instituto de Biotecnologia Aplicada à Agropecuária (Bioagro) da UFV. Esse laboratório é associado ao Instituto Nacional de Ciência e Tecnologia (INCT) em Interações Planta-Praga, coordenado pela professora Elizabeth Fontes. O estudo gerou a publicação de artigos em periódicos importantes, dentre eles a Frontiers in Plant Science, referência na área de biologia molecular de plantas, e a Scientific Reports, que integra o grupo Nature. Além disso, virou capítulo de livros e recebeu menção honrosa no International Symposium on Plant Molecular Genetics, promovido pela Sociedade Brasileira de Genética. Source: UFV and CAPES

AL 09 - Plant Transformation - Cotton Laboratory Activities - PlantStress Biotech INCT Searching for vital insect-pest genes/molecules (Helicoverpa armigera and Spodoptera frugiperda ), using large-scale sequencing of their transcriptome and in vitro validation of gene expression. S election of new Cry molecules with high toxic activity against S. frugiperda and H. armigera . In silico identification of target molecules against insects and nematodes, through the design of specific chemical drugs for the development of new insecticides and nematicides. Analysis of the genome of Meloidogyne spp. for the selection of genes/molecules vital for gall formation. Selection of potential genes involved in the resistance of contrasting genotypes (peanuts, soybean, rice, cotton, and coffee). Integration of monocotyledonous transcriptome data (rice, corn, wheat, Musa spp., and Sorghum spp.) submitted to water deficit, generated by mass sequencing (Illumina – HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes from Musa spp., Arachis spp., and cowpea submitted to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Use of qRT-PCR for validation of key genes expression from metabolic pathways related to plant responses to combined stresses (biotic-biotic, biotic-biotic, abiotic-biotic). Search and validation of novel regulatory sequences (promoters) responsive to biotic and abiotic stresses, in crop plants, using transient and stable transformation (soybeans, cotton, and maize). Sequencing and selection of plant small RNAs, mRNAs, and circular RNAs (Arachis spp. , Musa spp. , soybean, pitangueira, cashew tree) submitted to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function, potentially involved in nematode resistance mechanisms, through molecules overexpression or gene silencing (RNAi) strategies. Validation of nematode genes function, potentially involved in the parasitism mechanisms, through RNAi strategies in model systems. Assessment and monitoring technology of biotech assets prospected for intellectual protection. Development of universal vectors containing all prospected and patented genetic elements by different institutions from the INCT Project. National and international protection of genes and regulatory sequences, via patents. Developing of GM soybean, cotton, and maize plants through overexpression of molecules and/or gene silencing strategies for drought tolerance and nematode resistance. Developing of GM soybean, cotton, and corn plants through overexpressing Bt toxin and dsRNAs sequences applied to H. armigera and S. frugiperda control. Functional validation of multiple pyramided genes involved in multiple traits, including nematodes and insect-pests (S. frugiperda or H. armigera ) resistance and drought tolerance in GM soybean and cotton plants. Phenotyping (greenhouse and/or field simulation) GM maize, soybean, and cotton plants for drought tolerance and/or resistance to S. frugiperda , H. armigera and Meloidogyne spp. Laboratory Description​ The Plant-Pest Molecular Interaction Laboratory (LIMPP) is coordinated by Dr. Maria Fatima Grossi-de-Sa. Research interests include both basic and applied sciences, focusing on plant-pest molecular interactions (pathogens and insect-pests) to develop novel crop protection strategies, especially for cotton and soybean. The LIMPP group’s research is known for its expertise in plant biotechnology using functional genomics, notably working on biotechnological aspects of RNA interfering (RNAi) mechanism applied to insect-pests and phytonematodes. Other current research interests include exploring new technologies for plant genetic transformation and genome editing, novel regulatory sequences for genetic engineering of crop plants for protection against insect-pests and phytonematodes, drought tolerance, and development of recombinant proteins. Research Lines Search for novel genes/molecules and peptides to be applied on the control of cotton and soybean insect-pests. Search for target genes/molecules of plant parasitic nematodes (Meloidogyne spp., Rotylenchus reniformis , Aphelenchoides spp.) to be applied in gene silencing approaches. Genetic engineering of crop plants using genome editing technologies and target genes/molecules of contrasting soybean genotypes (resistant/susceptible to Meloidogyne spp.). Search for genes/molecules and small RNAs in pest-resistance and drought-tolerant plant genotypes potentialy involved in the response to biotic and abiotic stresses (cross-stress). Validation of novel prospected biotech assets using overexpression of molecules or gene silencing approaches in plant models. Genetic engineering development of crop plants (cotton, soybean, sugarcane) using molecules/dsRNAs overexpression, gene silencing, and genome editing approaches for traits that include pest control, increase in biomass, and drought tolerance. Recent Publications ATELLA, A. L.; Grossi-de-Sa, M. F.; Alves-Ferreira, M. (2023). Cotton promoters for controlled gene expression. Electronic Journal of Biotechnology, v. 62, p. 10.1016/j.ejbt. https://doi.org/10.1016/j.ejbt.2022.12.002 ​ ​ BASSO, M. F.; Lourenço-Tessutti, I. T.; Moreira-Pinto, C. E.; Mendes, R. A. G.; Pereira, D. G.; Grandis, A.; Macedo, L. L. P.; Macedo, A. F.; Gomes, A. C. M. M.; Arraes, F. B. M.; Togawa, R. C.; do Carmo Costa, M. M.; Marcelino-Guimaraes, F. C.; Silva, M. C. M.; Floh, E. I. S.; Buckeridge, M. S., de Almeida Engler, J., Grossi-de-Sa, M. F. (2023) . Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita . Plant Cell Report , v. 42, n. 1, p. 137-152. https://doi.org/10.1007/s00299-022-02941-3 ​ ​ FONSECA, F. C. A.; Antonino, J. D.; de Moura, S. M.; Rodrigues-Silva, P. L.; Macedo, L. L. P.; Gomes Júnior, J. E.; Lourenço-Tessuti, I. T.; Lucena, W. A.; Morgante, C. V.; Ribeiro, T. P.; Monnerat, R. G.; Rodrigues, M. A.; Cuccovia, I. M.; Mattar Silva, M. C.; Grossi-de-Sa, M. F. (2023) . In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer. Bulletin of Entomological Research, v. 8, p. 1-12. https://doi.org/10.1017/S000748532200061X ​ MOREIRA, V. J. V.; Pinheiro D. H.; Lourenço-Tessuti, I. T.; Basso, M. F. ; Lisei-de-Sá, M. E.; Silva, M. C. M.; Danchin, E. G. J. ; Guimarães, P. M.; Grynberg, P.; Brasileiro, A. C. M.; Macedo, L. L. P.; Morgante, C. V.; Engler, J. A.; Grossi-de-Sá, M. F. (2023) . In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. Journal of Pest Science , v. 1, p. 1. https://doi.org/10.1007/s10340-023-01623-7 ​ NIZOLLI, V. O.; Oliveira, V. F.; Maia, L. C.; Pegoraro, C.; Oliveira, A. C. (2023) . Genome editing in rice: New paths for an old crop. Perspectives In Agriculture, Veterinary Science, Nutrition And Natural Resources, v. 2023, p. 1-8. http://dx.doi.org/10.1079/cabireviews.2023.0008 ​ PEREIRA, B. M.; Arraes, F.; Martins, A. C. Q.; Alves, N. S. F.; Melo, B. P.; Morgante, C. V.; Saraiva, M. A. P.; Grossi-de-Sá, M. F.; Guimarães, P. M.; Brasileiro, A. C. M. (2023). A novel soybean hairy root system for gene functional validation. PLoS One, v. 18, p. e0285504. https://doi.org/10.1371/journal.pone.0285504 ​ TRENZ, T. S.; Turchetto-Zolet, A. C.; Margis, R.; Margis-Pinheiro, M.; Maraschin, F. S. (2023) . Functional analysis of alternative castor bean DGAT enzymes. 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FIRMINO, A.A.P.; Pinheiro, D.H.; Pinto, C.E.M.; Antonino, J.D.; Macedo, L.L.P.; Martins-de-Sa, D.; Arraes, F.B.M.; Coelho, R.R.; Fonseca, F.C.A.; Silva, M.C.M.; Almeida-Engler, J.; Silva, M.S.; Lourenço-Tessutti, I.T.; Terra, W.R.; Grossi-de-Sa, M.F. (2020) . RNAi-mediated suppression of Laccase2 impairs cuticle tanning and molting in the cotton boll weevil (Anthonomus grandis ). Frontiers in Physiology , v. 11, p. 591569. https://doi.org/10.3389/fphys.2020.591569 . GRYNBERG, P.; Togawa, R.C.; Freitas, L.D.; Antonino, J.D.; Rancurel, C.; Costa, M.M.C.; Grossi-de-Sa, M.F.; Miller, R.N.G.; Brasileiro, A.C.M.; Guimaraes, P.M.; Danchin, E.G.J. (2020) . Comparative genomics reveals novel target genes towards specific control of plant-parasitic nematodes. Genes , v. 11, p. 1347. https://doi.org/10.3390/genes11111347 . ​ ​ IBARRA, L.N.; Alves, A.E.O.A.; Antonino, J.D.; Prado, G.S.; Pinto, C.E.M.; Soccol, C.R.; Vasconcelos, E.A.R.; Grossi-de-Sa, M.F. (2020) . Enzymatic activity of a recombinant β-1,4-endoglucanase from the cotton boll weevil (Anthonomus grandis ) aiming second generation ethanol production. Scientific Reports , v. 10(1), p. 5367. https://doi.org/10.1038/s41598-019-56070-1 . ​ ​ MOTA, A.P.Z.; Fernandez, D.; Arraes, F.B.M.; Petitot, A.S.; Paes-Melo, B.; Lisei-de-Sa, M.E.; Guimaraes, P.M.; Brasileiro, A.C.M.; Albuquerque, E.V.S.; Danchin, E.G.J.; Grossi-de-Sa, M.F. (2020) . Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics. Molecular Genetics and Genomics , v. 295, p. 1063-1078. https://doi.org/10.1007/s00438-020-01677-7 . MOURA, S.M.; Rossi, M.L.; Artico, S.; Grossi-de-Sa, M.F.; Martinelli, A.P.; Alves-Ferreira, M. (2020) . Characterization of floral morphoanatomy and identification of marker genes preferentially expressed during specific stages of cotton flower development. Planta , v. 252(4), p. 71. https://doi.org/10.1007/s00425-020-03477-0 . ​ ​ NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Grossi-de-Sa, M.F. (2020) . Transcriptome analysis and knockdown of the juvenile hormone esterase gene reveal abnormal feeding behavior in the sugarcane giant borer. Frontiers in Physiology , v. 11, p. 588450. https://doi.org/10.3389/fphys.2020.588450 . NORIEGA-VASQUEZ, D.D.; Arraes, F.B.M.; Antonino, J.D.; Macedo, L.L.P.; Fonseca, F.C.A.; Togawa, R.C.; Grynberg, P.; Silva, M.C.M.; Negrisoli, A.S.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020) . Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source. PLoS One , v. 15(8), p. e0235575. https://doi.org/10.1371/journal.pone.0235575 . PAES-MELO, B.; Lourenço-Tessutti, I.T.; Morgante, C.V.; Santos, N.C.; Pinheiro, L.B.; Jesus-Lins, C.B.; Silva, M.C.M.; Macedo, L.L.P.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020) . Soybean embryonic axis transformation: combining biolistic and Agrobacterium -mediated protocols to overcome typical complications of in vitro plant regeneration. Frontiers in Plant Science , v. 11, p. 1228. https://doi.org/10.3389/fpls.2020.01228 . PAES-MELO, B.; Lourenço-Tessutti, I.T.; Paixao, J.F.R.; Noriega-Vasquez, D.D.; Silva, M.C.M.; Almeida-Engler, J.; Fontes, E.P.B.; Grossi-de-Sa, M.F. (2020) . Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit. Scientific Reports , v. 10(1), p. 16231. https://doi.org/10.1038/s41598-020-72464-y . RIBEIRO, T.P.; Basso, M.F.; Carvalho, M.H.; Macedo, L.L.P.; Silva, D.M.L.S.; Lourenço-Tessutti, I.T.; Oliveira-Neto, O.B.; Romano, E.; Lucena, W.A.; Silva, M.C.M.; Tripode, B.M.D.; Abreu-Jardin, T.P.F.; Miranda, J.E.; Alves-Ferreira, M.; Morgante, C.V.; Grossi-de-Sa, M.F. (2020) . Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. Biotechnology Research & Innovation , v. 3, p. 15. https://doi.org/10.1016/j.biori.2019.12.003 . SANTOS, C.; Nogueira, F.C.S.; Domont, G.B.; Fontes, W.; Prado, G.S.; Habibi, P.; Santos, V.O.; Oliveira-Neto, O.B.; Grossi-de-Sa, M.F.; Jorrín-Novo, J.V.; Franco, O.L.; Mehta, A. (2020) . Proteomic analysis and functional validation of a Brassica oleracea endochitinase involved in resistance to Xanthomonas campestres . Frontiers in Plant Science , v. 11, p. 201. https://doi.org/10.3389/fpls.2019.00414 . Our Team Maria Fatima Grossi-de-Sa Team Leader Maria Fatima Grossi-de-Sa holds a bachelor's degree in Biological Sciences - biomedicine modality from the University of Brasilia (1979), a master's degree in Biological Sciences (Molecular Biology) from the University of Brasilia (1982), a doctorate (Doctorat Et Sciences) in Molecular Biology from the Université Paris VII-France (1987), and was a postdoctoral fellow at the Plant Genetic System-Ghent-Belgium (1988) and at the University of California in San Diego (1995-1996). She is the Lead Researcher at EMBRAPA Genetic Resources and Biotechnology (since 1989) and professor at the Catholic University of Brasília (since 2004). She is a CNPq productivity fellow (level 1A), member of CAPES International Advisory Committee (since 2007), full member (Agrarian Sciences) of the Brazilian Academy of Sciences (elected in 2011) and a member of the World Academy of Science -TWAS (elected in 2014). Among other awards and honors, she notably received the Scopus Award 2010 (Elsevier / CAPES) and the medal of the National Order of Scientific Merit (2018). She held the position of coordinator at the Biotechnology area and alternate member of CTC-ES at CAPES (2007-2014), and the presidency of the Brazilian Society of Biotechnology - SBBiotec (2008-2013 and 2016-current). She has experience in the field of Plant Genetics and Biotechnology, with an emphasis on Genetic Engineering and Plant Molecular Biology. The primary focus of her research is on the development of biotechnological products, using different strategies, including genome editing, aiming to increase tolerance and resistance to biotic and abiotic stress in plants. Morevoer, biotechnological tools are applied for the development of biopharmaceuticals. Her main research fields include: plant defense proteins, insecticidal proteins, plant-pest molecular interaction, and biotechnological assets applied to agribusiness. Carolina Vianna Morgante She is undergraduated in Biological Sciences at University of Sao Paulo - Biosciences Institute (1999). Carolina Morgante holds a master's and doctorate's degrees in Agronomy (Genetics and Plant Breeding) from the University of São Paulo (2003 and 2008, respectively). She is currently a researcher at Embrapa Semiárido and has experience in Genetics, focusing on Plant Genetics and Molecular. Diana Isolda Clotilde Fernandez She is currently a permanent Senior researcher at the French Research Institute for Development - Institut de Recherche pour le Développement (IRD, France) and remained until November 2020 at Embrapa-Cenargen. She has experience in Biochemistry, with emphasis on Molecular Biology, working on the following subjects: phytopathology, plant-pathogen interactions, plant immunity, nematodes, rust, rice, Coffea arabica , Hemileia vastatrix , Meloidogyne spp. Isabela Tristan Lourenço Tessutti Isabela is undergraduated in Biological Sciences from the University of Brasilia (2006), and holds a master's and a doctorate's degree in Molecular Biology from the same University (2008 and 2014, respectively). She was a post-doctoral fellow (2020) at the Institut National de Recherche pour l'Agriculture , l'Alimentation et l'Envrionment (INRA - Sophia Antipolis/France). Recently, she works at the Plant-Pest Interaction Laboratory at Embrapa Genetic Resources and Biotechnology, coordinated by Dr. Maria Fatima Gross-de-Sa. Her main research fields are: plant-pest interaction, plant resistance to biotic stress (nematodes and insects), and tolerance to abiotic stress (drought). She has expertise in: functional genomics for phytonematodes, insects, plants and bacteria; plant genetic transformation; gene (RNAi) silencing; heterologus expression of proteins using bacterial cells; functional characterization of plant promoters; spacial and temporal determinarion of gene expression using real-time PCR; genome editing using CRISPR methodology for biomass increase, drought tolerance and pest resistance. Leonardo Lima Pepino de Macedo Leonardo is undergraduated in Biological Sciences from the Federal University of Rio Grande do Norte (2005). He holds a master degree in Biochemistry from the same University (2007) and a doctorate's degree in Genomic Sciences and Biotechnology from the Catholic University of Brasília (2012). He has experience in Biochemistry and Molecular Biology, with expertise in the following areas: cloning and expression of proteins in heterologous systems; bioprospecting proteins with entomotoxic activity (vicillins, lectins, proteinase inhibitors and Cry toxins) aiming at the control of dipterous, lepidopteran and coleopteran insects; development of gene silencing strategies via RNAi for the control of insect pests. Maria Eugenia Lisei de Sa Maria Eugenia is undergraduated in Biological Sciences from Faculdades Metodistas Integradas Isabela Hendrix (1981), Master in Agronomy (Phytotechnics) from the Universidade Federal do Ceará (1984), PhD in Genetics and Biochemistry from the Universidade Federal de Uberlândia (2004) and post-doctorate fellow in Biotechnology at the Institute de Recherche pour le Développement-França (2013). She is Researcher (II) at the Minas Gerais Agricultural Research Corporation (EPAMIG) and currently works as a collaborative researcher at Embrapa Genetic Resources and Biotechnology -Cenargen. He has experience in the field of soybean breeding, with an emphasis on the development of soybean cultivars with characteristics suitable for human consumption. Her expertise lies on plant defense proteins (proteinase inhibitors, alpha-amylase inhibitors, lectins, defensins, osmotins); plant-pest molecular interaction; development of genetically modified plants for resistance to biotic stress (insects and nematodes) and tolerance to abiotic stress. Maria Cristina Mattar da Silva Maria Cristina is undergraduated in Biological Sciences from the Universidade Estadual Paulista Júlio de Mesquita Filho (1984) and from Universidade de Brasília (1987). She holds a master's degree in Biological Sciences (Molecular Biology) from the Universidade de Brasília (1992) and a doctorate's degree in Biological Sciences (Molecular Biology) from University of Brasília (2002). She is a Researcher at Embrapa Genetic Resources and Biotechnology since 1989. She is expert in plant molecular biology, working in the field of plant biotechnology for biotic and abiotic stress. The main focus of her researches are: evolution of molecules in vitro for selection of variants with improved activity; molecular studies of plant-pest interaction for insect resistance. Currently, she is a Member of the Brazilian Society of Biotechnology. FIND OUT MORE ABOUT OUR TEAM Contact Maria Fatima Grossi de Sá EMBRAPA Genetic Resources and Biotechnology W5 Norte Avenue (end) - P.O. Box 02372 - Postal Code 70770-917 - Brasília, DF - Brazil E-mail: fatima.grossi@embrapa.br Phone number: +55 61 3448-4705

Acerca de About the Project Biological systems research focusing on elucidating plant stress mechanisms and signaling pathways is important for developing resistant cultivars in a climate change scenario. Pathogen attacks and abiotic stresses such as water deficit are essential during plant development, and the identification of genes and molecules, which regulate resistance responses, are critical to develop crop cultivars capable of doing in the face of these stresses. In association with abiotic stresses, plants must also defend themselves against pest’s attacks, including fungi, bacteria, insect pests, and nematodes. Insect-pests comprise the most serious factor affecting productivity losses in the Brazilian agribusiness. Losses in soybean, cotton, and corn caused by the caterpillars Helicoverpa armigera , Spodoptera frugiperda , and phytonematodes constitute the most critical phytosanitary problem in terms of competitiveness for the commodities production and exportation. Faced with scenario cases involving plant resistance breakdown, environmental association of biotic and abiotic stresses, fewer characterized and introgressed genes, and higher complexity of plant responses have been reported. All these reports demonstrate the importance of the research towards the identification of genes/molecules involved in pest resistance and water deficit responses. In this context, the National Institute of Science and Technology, PlantStress Biotech INCT, integrates various Brazilian research groups and international partners, experts in plant physiology, transcriptomic, epigenetic, proteomic, bioinformatic and functional genomics analyses. The integrative research group represents a multidisciplinary and multi-institutional network with national and international excellence to generate innovative biotechnologies applied to corn, soybean and cotton focused on the tolerance to deficit hydric and pest control (Meloidogyne spp, H. armigera and S. frugiperda ). The project includes bioprospection, isolation, characterization, and functional validation of genes/molecules involved in plant pest resistance and drought tolerance. The PlantStress Biotech INCT action comprises: ​ Prospection for genes and molecules involved in resistance/tolerance to specific and combined stresses in native plants and resistant/tolerant contrasting genotypes, through the elucidation of the stress mechanisms and signaling pathways; Prospection for genes and molecules involved in the defense and parasitism of target pests; Search for promoters responsive to pests and water deficit, and small RNAs involved in the regulation of target genes; Use different strategies (gene/molecules overexpression or gene silencing) for biological functional validation in model systems; Developing of new nanoencapsulated insecticides/nematicides from validated molecules; Developing of biotechnological products (concept proof) in GM crop plants (cotton, soybean, and maize); Evaluation of developed GM crop plants in simulated field experiments. ​ The biotech assets generated will be applied to the development of biotechnology products, including nanobioinsecticides and genetically modified crops (soybean, maize, and cotton) against biotic and abiotic stresses. In addition to the biotech assets obtained and characterized in this project, an understanding of molecular and physiological factors related to drought tolerance and their interaction with pests and other environmental stresses will contribute to prevent, unravel and adapt to climatic changes. Alongside its scientific agenda, the PlantStress Biotech INCT group, formed by researchers from five Embrapa units and five Federal Universities (UnB, UFRJ, UFRGS, UFC, and UFPel), and collaborators from the public and private sectors, concentrate their efforts on training students and professionals in different and update approaches involving biotechnology, genomics, and bioinformatics. Actions will also strengthen joint action and the internationalization of undergraduate and graduate training engaged in PlantStress Biotech INCT research, and to increase the visibility and international insertion of its teams. The PlantStress Biotech INCT network focuses not only on the generation of biotech assets applied to drought and pests in soybean, cotton, and maize but also on other important agronomic traits (seed and fruit quality, an increase of biomass and nutritional value, among other) and in other crops important for Brazilian agribusiness.

AL 12 - Colaboration Lab Laboratory Activities - PlantStress Biotech INCT Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Carmen S. Soares Pires Team Leader Dr. Carmen is undergraduated in Biology from the Federal University of Viçosa (1984), holds a master's degree in Entomology from the same university and a Ph.D in Biology fomr the Northern Arizona University (1998). She has been a researcher at Embrapa Genetic Resources and Biotechnology since 1989. She has experience in Ecology, with amphasis on Insect Ecology, working mainly o the following topics: population dynamics, plant-insect pest-natural enemies interaction, conservative biological control and pollination in agroecosystems. Since 2003, she has been involved in environmental risk analysis projects of stressors on non-target organisms (predatory insects, pollinators and non-target herbivores). Angharad MR Gatehouse Team Leader Prof Angharad Gatehouse has the Chair of Invertebrate Molecular Biology at Newcastle University and is Director of Expertise for BioEconomy. Her research focuses on the molecular and biochemical bases of plant-pest interactions with a view to developing novel strategies for crop protection. Her group was one of the first to produce insect-resistant transgenic crops. More recently her group has been using functional genomics to better understand the molecular responses of crops to biotic stress (pathogens and insect pests) for the development of molecular markers. In collaboration with Durham University, her group are actively involved in developing novel biopesticides including those based on RNAi. In addition to their efficacy, the group is also involved in the biosafety of these strategies, notably in terms of their potential effects on beneficial insects such as pollinators and natural enemies. She has published extensively in the field, including in discovery journals (Nature, PNAS). Contact Carmen S. Soares Pires Embrapa - Recursos Genéticos e Biotecnologia Parque Estação Biológica - PqEB, s/n, Brasília - DF carmen.pires@embrapa.br +55 61 3448-4433 ​ Angharad MR Gatehouse School of Natural and Environmental Sciences - Newcastle University, Ridley Building. a.m.r.gatehouse@ncl.ac.uk +44 (0) 191 208 8838 ​ ​

AL 01 - Genomics and Proteomics Laboratory Activities - PlantStress Biotech INCT Integration data from monocotyledon transcriptome (rice, corn, wheat, Musa and sorghum) submitted to water deficit, generated by mass sequencing (Illumina - HiSeq) in previous projects. Integration of transcriptome data from drought-tolerant genotypes of Musa spp., Arachis spp. and cowpea subjected to water deficit combined with biotic stress (Meloidogyne spp. or Mycosphaerella ) in bioassays. Integration and sequencing of transcripts of fungus-tolerant genotypes in environments subjected to multiple stresses (Musa X Mycosphaerella musicola and Fusarium oxysporun ). Validation of key genes expression in the metabolic pathways of plants' response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Small RNAs, mRNAs and circular plant RNAs sequencing from Arachis , Musa , soybean, pitangueira, and cashew, previously subjected to biotic and/or abiotic stresses, using the Illumina platform. Validation of plant genes function potentially involved in drought tolerance mechanisms in Arabidopsis , rice or Setaria plants by overexpression or silencing strategies. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description​ The Genomics and Proteomics Laboratory studies the prospection and identification of new genes, regulatory sequences and molecules involved with the resistance/tolerance responses of plants to biotic and abiotic stresses, aiming at the elucidation of the molecular mechanisms of plants that occur in response to different stresses, combined or not. The research group also performs fucntion validation of these assets in model plants. Research Lines Prospecting genes/molecules of interest for pest and drought control in wild germplasm of Musa spp. Prospecting target molecules in phytoparasitic nematodes (Meloidogyne spp.) and phytopathogenic fungi (Pseudocercospora spp.) for pest control​. Prospecting for small RNAs in plant genotypes that are resistant/tolerant to pests and drought that may be involved in responding to these stresses​. Prospecting for genes/molecules that are efficient in pest control and tolerance to water deficit simultaneously (cross-stress).​ Validation of prospective innovation assets by their overexpression or gene silencing in model plants for analysis and validation of their function​. Robert Neil Gerard Miller Team Leader Graduated in Biological Sciences - Manchester Metropolitan University, UK (1990), Master in Plant Protection - University Of Bath, UK (1991) and PhD in Molecular Biology and Phytopathology - University of Reading, UK (1995). He is currently Associate Professor I at the University of Brasilia (Campus Darcy Ribeiro, Department of Cellular Biology), supervising the Graduate Programs in Molecular Biology, Phytopathology and Microbial Biology. He worked between 2014 and 2016 as Coordinator of the Graduate Program in Molecular Biology (CAPES concept 6). He works as editor for the journals Annals of Botany and Tropical Plant Pathology, he is coordinator of national and international projects, mainly in the following themes: functional genomics of plants and microorganisms; search for genes for resistance to biotic stress in plants; and characterization of phytopathogenic and mycotoxigenic fungi. Contact Robert Neil Gerard Miller Microbiology Laboratory: Plant-Prague Interaction, Bloco I-1-35/8, Institute of Biological Sciences, Department of Cell Biology, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Postal Code 70910-900, Brasília, DF, Brazil.

< Back Research Developing Nematode Control Alternatives Receives Awards at Conference Students Affiliated with INCT Plant Stress Biotech, Developing Nematode Control Alternatives, Win Best Research Awards at the 53rd Brazilian Phytopathology Congress. Nematodes have a significant impact on agriculture by attacking plant roots, disrupting their growth and nutrient uptake. These parasites can cause substantial damage in various crops, including banana and cotton. In the banana crop, for instance, nematodes can weaken plants, displaying symptoms like yellowing, wilting, and even premature death of seedlings. In cotton, these organisms can induce the formation of root galls, impairing water and nutrient absorption, leading to reduced productivity and fiber quality. Cotton Plant Roots Infected by Nematodes. The Marked Areas Are Galls, Structures Formed as a Result of Infection by the Nematode M. incognita. Effectively controlling nematodes remains a constant concern for farmers, as inadequate management can result in significant harvest losses. Techniques like crop rotation, cover cropping, and application of nematicide chemicals are some of the approaches used to mitigate damage caused by these parasites. However, a more sustainable and promising approach is the development of plants resistant to nematodes. Through biotechnology techniques, it's possible to improve the resistance genes into target crops, granting them the ability to better withstand nematode attacks. This approach not only reduces reliance on chemical agents but can also boost crop productivity and quality, thereby contributing to food security and agricultural economies. Best Research in the Undergraduate and Master's Categories Between August 7th and 10th, 2023, the 53rd Brazilian Phytopathology Congress took place in Brasília, Federal District. The event aims to bring together professionals from the fields of education, research, and extension in both the public and private sectors, as well as students from Brazil and abroad, along with experts from various segments of production chains. The Conference objective is to discuss the current status and scientific advancements related to plant diseases and their control methods, fostering knowledge exchange and anticipating new challenges to be overcome. Bringing innovations and promising outcomes to agriculture, Sara Vitorino da Rocha Lemes , a Biotechnology student, and Lucas Santos Bastos , an Agronomist and Master's candidate in the Phytopathology Postgraduate Program, both from the University of Brasília - UnB, were awarded for their research. Sara earned second place in the Undergraduate category, while Lucas secured third place in the Master's category. Sara's work, titled "Development of cotton plants with reduced susceptibility to Meloidogyne incognita through RNA interference". The aim of Sara's work is to develop transgenic cotton plants that produce a specific RNA molecule for nematode control. Therefore, when the parasite feeds on the plant, it also ingests this RNA, hampering its development and reducing the number of galls and eggs in the plant roots. Lucas's master's research aimed to identify and characterize genes associated with resistance to M. incognita and tolerance to water stress, as well as how the interaction between these two stresses occurs in banana plants. Through this study, it becomes possible to enhance our understanding of the mechanisms linked to these stresses and provide data for banana genetic improvement. It was with the work titled "Differential Expression of Genes Associated with Meloidogyne incognita Infection and Water Deficit in Musa acuminata " that he secured third place in his category. These awards showcase the quality and effectiveness of projects affiliated with INCT Plant Stress Biotech, as well as one of INCT's objectives in developing biotechnological tools for the Brazilian agribusiness.

Events Organization Workshops with the INCT-PlantStress-Biotech team , held in February/2022. ​ ​ Member of international committee XXVIII Plant and Animal Genome Conference , San Diego, CA, EUA, Antônio C. de Oliveira, held from 11 to 18 January 2020. ​ Member of international committee ISRFG 2019 , Taipei, Taiwan, Antônio C. de Oliveira, held from 4 to 6 November 2019. ​ Workshops with the INCT-PlantStress-Biotech team , held in April/2017, December/2018 and May/2019. Organization of the 7th Brazilian Congress of Biotechnology , organized by SBBIOTEC (Brazilian Society of Biotechnology), whose current president is Dr. Maria Fatima Grossi-de-Sá, held from 18 to 21 November 2018.

Associated Laboratories (ALs) AL 01- Genomics and Proteomics Team Leader: Robert N. G. Miller (UnB) Partner Institutions: UnB, Embrapa Cenargen AL 02 - Transcriptomics, Epigenetics and Functional Genomics Team Leader: Rogério Margis (UFRGS) ​ Partner Institutions: UFRGS, UFRJ, Embrapa Cenargen AL 03 - Molecular Genetics Team Leader: Márcio Alves-Ferreira (UFRJ) ​ Partner Institutions: UFRJ, Embrapa Cenargen AL 04 - Molecular Physiology Team Leader: Joaquim A. G. Silveira (UFC) ​ Partner Institutions: UFC, UFPel, Embrapa Clima Temperado AL 05 - Plant-Pest Interaction Team Leader: Patrícia Messenberg Guimarães (Embrapa Cenargen) ​ Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Cerrados, Embrapa Arroz e Feijão, Embrapa Milho e Sorgo AL 06 - Bioinformatics Roberto Coiti Togawa (Embrapa Cenargen) ​ Partner Institutions: Embrapa Cenargen, UnB, UFPel AL 07 - Insects-Nematodes: Creation and Bioassays Team Leader: Leonardo Pepino (Embrapa Cenargen) ​ Partner Institutions: Embrapa Cenargen, Embrapa Soja, Embrapa Milho e Sorgo AL 08 - Plant Transformation - Soybean Team Leader: Maria Helena Zanettini (UFRGS) ​ Partner Institutions: UFRGS AL 09 - Plant Transformation - Cotton Team Leader: Maria Fátima Grossi-de-Sa (Embrapa Cenargen) ​ Partner Institutions: Embrapa Cenargen AL 10 - Plant Transformation - Corn Team Leader: Newton Carneiro (Embrapa Milho e Sorgo) ​ Partner Institutions: Embrapa Milho e Sorgo AL 11 - Biometrics Team Leader: Antônio C. de Oliveira (UFPel) ​ Partner Institutions: UFPel AL 12 - Evaluation of Environmental Risks Carmen Pires (Embrapa Cenargen) Angharad Gatehouse (Newcastle University) ​ ​ ​ Partner Institution: Embrapa Cenargen e Newcastle University AL 13 - Field Phenotyping - Public Sector Team Leader: Jaime Cavalcanti (Embrapa Algodão) ​ Partner Institutions: Embrapa Soja, Embrapa Milho e Sorgo, Embrapa Algodão, Embrapa Clima Temperado AL 14 - Field Phenotyping - Private Sector Team Leader: Rafael Galbieri (IMAmt) ​ Partner Institutions: IMAmt - Instituto Matogrossense do Algodão AL 15 - Molecular Plant-Pathogen Interaction Team Leader: Francismar C. Marcelino Guimarães (Embrapa Soja) ​ Partner Institutions: Embrapa Soja AL 16 - Biotechnological Applications of Microorganisms Team Leader: Maite Vaslin de Freitas Silva (UFRJ) ​ Partner Institutions: Federal University of Rio de Janeiro