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< Back INCT PlantStress Biotech’s Participation in the International Congress of Nematology (ICN) in Antibes Juan-Les-Pins, France Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech , participated in the 7th International Congress of Nematology (ICN) in Antibes Juan-Les-Pins, France, between the 1st and 6th of May 2022. As an invited speaker in the omics session, he delivered a lecture entitled “Analysis of the banana root transcriptome in response to root-knot nematode infection and water deficit” with focus on results generated in the INCT project. Drought and nematodes are constraints to global agriculture that can occur simultaneously. Banana ( Musa spp.), whilst among the world’s most widely consumed fruits, is susceptible to both drought stress as well as infection by the endoparasitic root-knot nematode (RKN) Meloidogyne incognita . Data from a transcriptome analysis of the responses to RKN, drought and combined stresses in a drought resistant Musa acuminata genotype from the Embrapa breeding program was presented. The ICN conference provided a forum for presentation and discussion on the state of the art of nematology, with the participation of scientists from more than 55 countries worldwide - https://www.alphavisa.com/icn/2020/index.php .

INCT Partnership The partnerships of INCT PlantStress Biotech have proven to be essential for its progress. These partnerships essentially originate from the network of collaborations that its members have in various areas of operation. Private Companies Companies collaborating for the development of research projects Read More Graduate Courses Graduate Courses with the participation of leading INCT PlantStress Biotech researchers Read More Associated Labs Laboratories that comprise the INCT PlantStress Biotech Read More International Collaborators International Universities and Research Institutes that collaborate in INCT PlantStrss Biotech Read More Private Companies Partner companies involved in the development of projects for the INCT PlantStress Biotech CORTEVA Support in research for the development of new soybean genotypes. IMAmt Support in research for the development of new cotton genotypes. Bio Bu reau Support startups to formalize their business and develop their company. SEMPRE SEMENTES Support in research for the development of new soybean genotypes. TOLVEG Applied research with microorganisms and plant bio-stimulating substances. SoluBio Ensure sustainability and produce more with less. HAPISEEDS Support in analyze level of positive plant interaction with bioinoculants. ABRAPA Support in research for the development of new cotton genotypes. PEPBioLabs Enzymes and peptides for laboratories and companies. INL Performing interdisciplinary research and deploy and articulating nanotechnology for the benefit of society. Graduate Courses Graduate courses, whose partnership with INCT PlantStress Bioech allows the use of infrastructure, resources, and themes for training development, both at the undergraduate and postgraduate levels. Thus, in addition to its scientific and technological character, INCT PlantStress Biotech trains human resources at all levels, either through postgraduate courses in which its researchers are involved or through scholarships for scientific initiation, technological development, and postdoctoral studies (from Capes, CNPq, and FAPs). Postgraduate courses with participation of lead researchers from INCT PlantStress Biotech: Graduate course in Genomic Sciences and Biotechnology - Universidade Católica de Brasília Graduate course in Molecular Biology - Universidade de Brasília Graduate course in Biotechnology - Universidade Estadual de Londrina Graduate course in Biological Sciences (Molecular Biology) - Universidade de Brasília Graduate course in Sciences (Microbiology) - Universidade Federal do Rio de Janeiro Graduate course in Vegetal Biotechnology and Bioprocess - Universidade Federal do Rio de Janeiro, Graduate course in Plant Biotechnology - Universidade Federal do Rio de Janeiro Graduate course in Biotechnology and Biodiversity - Universidade de Brasília Graduate course in Cellular and Molecular Biology - Universidade Federal do Rio Grande do Sul Graduate course in Biotechnological Processes - Universidade Federal do Paraná Graduate course in Biological Sciences (Genetics) - Universidade Federal do Rio de Janeiro Graduate course in Phytopathology - Universidade de Brasília Alan Buddie - CABI; Bakeham Lane; Egham; Surrey TW20 9TY; UK – E-mail: a.buddie@cabi.org Ana Zotta Mota - Centre de Recherche Développement Nestlé, 101 Av. Gustave Eiffel, 37390 Notre-Dame-d'Oé, France – E-mail: anazottamota@gmail.com Angharad Gatehouse - School of Natural and Environmental Sciences, Newcastle University, Newcastle NE1 7RU, UK - E-mail: a.m.r.gatehouse@ncl.ac.uk David Bertioli - Department of Crop and Soil Science, University of Georgia, Athens, 30602 GA, USA - E-mail: bertioli@uga.edu Deepak Sharma - Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya (IGKV) - Raipur, 492012, India – E-mail: deepak1962@igkv.ac.in Diana Fernandez - IRD, CIRAD, Université de Montpellier, IPME, F-34398 Montpellier, France – E-mail: diana.fernandez@ird.fr Dirk Inze – Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium - E-mail: dirk.inze@psb.vib-ugent.be Etienne GJ Danchin - INRAE, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France – E-mail: etienne.danchin@inrae.fr Franc-Christophe Baurens - CIRAD, UMR AGAP Institut, F-34398 Montpellier, France – E-mail: franc-christophe.baurens@cirad.fr .  Gilbert Joseph Engler - INRAE, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France – E-mail: gilbertengler@gmail.com Henry Daniel - Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104-6030, USA - E-mail: hdaniell@upenn.edu Jan Leach - Department of Agricultural Biology, Colorado State University, Fort Collins, Colorado, USA – E-mail: jan.leach@colostate.edu Janice de Almeida Engler - Institut National de la Recherche Agronomique – INRAE, France – E-mail: janice.de-almeida@inrae.fr Juan Luis Jurat-Fuentes - Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA – E-mail: jurat@utk.edu Kazuo Nakashima – Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686, Japan - E-mail: kazuo.nakashima@affrc.go.jp Laurens Pauwels – Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium – E-mail: laurens.pauwels@psb.vib-ugent.be Lucia Colombo - Dipartimento di Bioscienze, Università Degli Studi di Milano, Via Celoria 26, 20133, Milan, Italy – E-mail: lucia.colombo@unimi.it Luis Willian Arge - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN 55108-6026, USA – E-mail: l.willianpacheco@gmail.com Martin Crespi - Université Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay, France - E-mail: martin.crespi@cnrs.fr Martin Kater - Department of Biosciences, Università degli Studi di Milano, via Celoria 26, 20133, Milan, Italy - E-mail: martin.kater@unimi.it Nelson Saibo - Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal – E-mail: saibo@itqb.unl.pt Nicolas Roux - Bioversity International, Parc Scientifique Agropolis II, Montpellier, France – E-mail: n.roux@cgiar.org Pat Heslop Harrison - University of Leicester, Department of Genetics and Genome Biology, Institute for Environmental Futures, Leicester LE1 7RH, UK – E-mail: pat.heslop-harrison@bbsrc.ac.uk Peggy Ozias - Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Tifton, GA, 31793, USA – E-mail: pozias@uga.edu Ping He - Department of Molecular, Cellular, & Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA – E-mail: pinghemi@umich.edu Randall Wisser - Institut National de la Recherche Agronomique – INRAE, France - E-mail: randall.wisser@inrae.fr Raquel Chan - Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional Del Litoral, Santa Fe, Argentina - E-mail: rchan@fbcb.unl.edu.ar Rod Wing - Center for Desert Agriculture, Biological and Environmental Sciences & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia – E-mail: rod.wing@kaust.edu.sa Shuangxia Jin - National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China - E-mail: jsx@mail.hzau.edu.cn Soraya Leal-Bertioli - Center for Applied Genetic Technologies, University of Georgia, Athens, 30602, GA, USA – E-mail: sorayab@uga.edu Todd Michael - The Plant Molecular and Cellular Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA - E-mail: tmichael@salk.edu Yiping Qi - Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland, USA – E-mail: yiping@umd.edu Zhiyong Wang - Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA – E-mail: zwang@carnegiescience.edu International Colaborators

Learn more about AL03, its main goals within the project and meet the laboratory's work team. AL 03 - Molecular Genetics Laboratory Activities - PlantStress Biotech INCT Integration of the transcriptome and sequencing of the model plant (Setaria) submitted to drought and high carbon dioxide content. Validate the expression of key genes in the metabolic pathways of plants response to combined stresses (biotic-biotic; biotic-biotic; abiotic-biotic) by qRT-PCR. Identify in silico promoters responsive to biotic and abiotic stress (drought and phytonematodes), with activity in different organs (root and leaf). Set up a vector bank with promoters directing the expression of reporter genes (GUS/GFP) in monocots and dicots. Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis , rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Márcio Alves Ferreira Team Leader Graduated in Biological Sciences with a BA in Genetics from the Federal University of Rio de Janeiro (1991), a PhD in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro/Ghent University (Brazil and Belgium/1997) and a Post-Doctorate in Genetics of plant development at the California Institute of Technology - Caltech (USA/2001). Full Professor at the Department of Genetics at the Institute of Biology at the Federal University of Rio de Janeiro. Has experience in plant genetics, with emphasis on Plant Biotechnology, working mainly on the following topics such as control of gene expression, abiotic stress and functional genomics. Accredited advisor in the postgraduate courses in Genetics (level 7) and in Plant Biotechnology and Bioprocesses (level 6) at UFRJ. He has supervised 25 Master's dissertations, 13 doctoral theses and 10 postdoctoral supervisions. Since 2010, he has been working as a member of the CAPES Biotechnology Area Committee in the postgraduate evaluation. Contact Marcio Alves-Ferreira Plant Molecular Genetics and Biotechnology Laboratory Bloco A, A2-93 Institute of Biology, Department of Genetics Universidade Federal do Rio de Janeiro, Cidade Universitária - CEP: 21941-617, Rio de Janeiro, RJ, Brazil.

Learn more about AL08, its main goals within the project and meet the laboratory's work team. AL 08 - Plant Transformation Laboratory Activities - PlantStress Biotech INCT Identify in silico promoters responsive to biotic and abiotic stress. Clone promoters responsive to drought and phytonematodes with activity in different organs (root and leaf). Validate promoters by transient transformation by biolistics or Agrobacterium rhyzogenes in target plants for in vivo testing of promoters (soybean, cotton and corn). Validate promoters by stable transformation in target plants for in vivo testing of promoters identified as promising in previous tests (soybeans, cotton and corn). Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Generate GM soybean, cotton and corn via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained regarding drought tolerance. Generate GM soybean and cotton via strategies of overexpression or silencing of plant genes and evaluate the phenotype obtained for resistance to nematodes. Generate GM soybean, cotton and corn via toxin overexpression strategies and evaluate the phenotype obtained regarding the control of Helicoverpa armigera and Spodoptera frugiperda . Laboratory Description Research efforts in our laboratory have focused on prospecting and characterization of genes involved in plant response to biotic and abiotic stresses. Emphasis: Soybean (Glycine max ), Jackbean (Canavalia ensiformis ) and Solanum nigrum . Approaches: Gene expression analysis; Gene silencing; Overexpression; Subcellular localization of gene products. For genetic transformation, we use mainly particle bombardment, but we are gaining experience on Agrobacterium -mediated transformation. Embryogenic tissues are used as targets for transformation. Research Lines Characterization of soybean genes involved in flood and drought tolerance. Overexpression of Jaburetox and Soyuretox, urease-derived peptides, for plant protection against insects, fungi and nematodes. Characterization of WRKY soybean genes responsive to water deficit and study of their promoters. • Expression of a gene that encodes an osmotin, isolated from Solanum nigrum, aiming enhanced drought tolerance in soybean. Our Team Maria Helena Bodanese Zanettini Team Leader She holds a degree in Natural History from the Federal University of Rio Grande do Sul (1972), a master's degree in Genetics and Molecular Biology from the Federal University of Rio Grande do Sul (1975) and a PhD in Genetics and Molecular Biology from the Federal University of Rio Grande do Sul ( 1982). Retired Full Professor at the Department of Genetics at UFRGS. She is currently Professor/Advisor of the Graduate Program in Genetics and Molecular Biology at the same University. Has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly in the lines of research: 1) Prospection of genes, tissue culture and genetic manipulation of plants; 2) Genetics, evolution and conservation of plants. Interested in the following topics: soy; somatic embryogenesis; anther culture; genetic transformation; biolistics; Agrobacterium system; co-transformation; transgenic plants; functional genomics; insect resistance; fungus resistance; genetic variability; population structure; plant cytogenetics; meiotic behavior; playback mode; fertility. Christian Bredemeier Graduated in Agronomy from the Federal University of Rio Grande do Sul (UFRGS), Master in Plant Science from UFRGS and PhD in Plant Nutrition/Precision Agriculture from the Technical University of Munich - Weihenstephan (Freising, Germany). Currently is Associate Professor at the Department of Crop Plants / UFRGS and Professor at the Graduate Program in Phytotechnics at UFRGS and at the Graduate Program in Precision Agriculture at the Federal University of Santa Maria (UFSM). Coordinates the Digital Agriculture Study Group (GEAD / UFRGS) and is a founding partner of the Brazilian Institute of Bioeconomy (INNBIO). Currently, is President of the Brazilian Commission for Precision and Digital Agriculture - CBAPD/MAPA (Period 2021-2023), Scientific Deputy Director of the Brazilian Association for Precision and Digital Agriculture - AsBraAP (Period 2020-2024), Member of the Chamber of Agro 4.0 and Member of the Network of Mentors in Innovation at ZENIT - Scientific and Technological Park of UFRGS. He was Coordinator of the Graduate Program in Phytotechnics / UFRGS, in the period 2017-2021. Areas of expertise: Precision Agriculture and Digital Agriculture, variable rate nitrogen fertilization, ecophysiology and crop plant management, sensors, remote sensing and unmanned aerial vehicles (UAVs). Contact Maria Helena Bodanese Zanettini Universidade Federal do Rio Grande do Sul Campus do Vale, Bento Gonçalves Avenue 9500, Building 4342212, Postal Code 91501-910, Porto Alegre, RS, Brazil E-mail: maria.zanettini@ufrgs.br Phone number: +55 51 3008-6725

Learn more about AL15, its main goals within the project and meet the laboratory's work team. AL 15 - Molecular plant-pathogen interaction Laboratory Description - PlantStress Biotech INCT In this INCT, Embrapa Soybean’s Plant Biotechnology Laboratory will act in the prospecting of genes involved in parasitism in nematodes, validation of identified genes via transient expression assays (compound soybean plants) and/or virus-induced gene silencing (VIGS), as well as resistance genes and defense mechanisms in soybean. Finally, it will assist in the molecular characterization of transformed soybean plants containing genes previously validated in model plants by AL 02 and AL 08, aiming at the identification of elite events, useful in the Genetic Improvement Program. We hope that the activities conducted at this research institute can contribute to the development of technologies for the sustainability of the soybean production chain, including genotypes adapted to different edaphoclimatic conditions and stresses that affect the crop. Research Lines Genetic and associative mapping of resistance genes to gall nematodes in soybean. Prediction and functional characterization of genes involved in nematode parasitism. Validate the function of plant genes potentially involved in resistance to nematodes via strategies of overexpression or silencing. Validate the function of nematode genes potentially involved in the parasitism mechanisms, via gene silencing strategies (RNAi) in model systems. Our Team Francismar C. Marcelino Guimarães Team Leader Francismar Correa Marcelino is a biologist graduated from the Federal University of Viçosa (2000), where he also took a master's and doctorate course in Genetics and Breeding (2006). She did her Postdoctoral (2011) in Molecular Plant Pathology at Iowa State University (US). She is a researcher at Embrapa Soja, working in the Molecular Genetics of Plants area, focusing on plant-pathogen molecular interaction, in the lines of transcriptomy research in response to infection by phytopathogens (P. pachyrhizi and nematodes), effector characterization and functional genomics in soybean. It also acts in the identification and validation of SNPs markers via genetic re-sequencing strategies for application in assisted selection for the development of soybean cultivars. She is a professor and advisor at the State University of Londrina in the postgraduate course in Biotechnology and Genetics, and in Bioinformatics at UFTPR. She has experience in the fields of Plant Molecular Genetics, Plant-Pathogen Molecular Interaction, Biochemistry and Biotechnology. She has supervised/co-supervised several students with scientific initiation, doctorate and postdoctoral degrees in the different lines of research she works with. Carlos Arrabal Arias Graduated in Agronomic Engineering (1988) and Master in Genetics and Breeding (1991) from the State University of Londrina and PhD in Agronomy (Genetics and Plant Breeding) from the Luiz de Queiroz School of Agriculture (1995). He is currently a researcher for the Brazilian Agricultural Research Corporation, at the National Agricultural Research Center-Embrapa Soybean. He is also a collaborating professor in the postgraduate course in Genetics and Molecular Biology at the State University of Londrina. He is currently a member of the Internal Technical Committee and the Internal Biosafety Committee of Embrapa Soja. Currently he is also the Leader of the National Soybean Improvement Project at Embrapa Soja, working mainly on the following themes: soybean cultivars, adaptability and stability, and genetic resistance to insect pests and diseases such as Asian rust. Ricardo Vilela Abdelnoor Researcher at the Brazilian Agricultural Research Corporation (EMBRAPA) since 1994, working in the field of Molecular Genetics and Soy Genomics. Graduated in Agronomy from the Federal University of Viçosa and doctorate in Agronomy (Genetics and Breeding) from the University of Nebraska, United States, in 2004. He currently participates in several research projects and led the multi-institutional projects "Technological Platform for Integrated Management of the Asian Rust of Soybean "(FINEP) and" GENOSOJA - National Consortium for Studies of the Soybean Genome "(CNPq), in addition to leading projects of Embrapa Macroprogram 2. He is currently an accredited Professor of the Genetics and Molecular Biology program at the State University of Londrina (UEL), having supervised and co-supervised master and doctoral students at the said university. He also serves as co-advisor at the Federal University of Viçosa (UFV), UNESP-Jaboticabal and State University of Maringá (UEM). He coordinated PROCISUR's "Functional Genomics" Platform for five years, and currently serves as a member of the International Soybean Genome Consortium and served as an elected member of the Soybean Genetics Committee (2009-2012). In 2009 he was elected to the "Continuing Committee" of the World Soybean Research Conference (World Soybean Research Conference) for the period 2009-2020, and in 2013 he was chosen as President of the "Continuing Committee", for the period 2013 -2020. He was President of the Organizing Committee of the VI Brazilian Soybean Congress (CBSoja 2012). He was a member of the National Technical Commission on Biosafety (CTNBIO) from 2012 to 2014. In the management area, he was Executive Secretary of the Internal Technical Committee (CTI) of Embrapa Soybean, from 2010 to 2013 and since 2013 he is the Deputy Head of Research & Development Embrapa Soybean. Since 2013 he is a member of the Sectorial Chamber of the Soybean Productive Chain, linked to the Ministry of Agriculture. Mauricio Conrado Meyer Graduate in Agronomy at State University of Ponta Grossa (1987), MSc in Phytopathology at University of Brasilia (1990) and PhD in Plant Protection at Sao Paulo State University Julio de Mesquita Filho (2001). Senior researcher at Embrapa Soybean (since 1989). Has experience in plant pathology, focusing on management of soybean diseases and breeding soybean cultivars for disease resistance. Contact Francismar C. Marcelino Guimarães EMBRAPA Soybean Campus Carlos João Strass Road (Orlando Amaral Access) - P.O. Box 231 - Postal Code 86001-970 - Londrina, PR - Brazil E-mail: francismar.marcelino@embrapa.br Phone:+55 43 3371-6000

Learn more about AL02, its main goals within the project and meet the laboratory's work team. AL 02 - Transcriptomics, Epigenetics and Functional Genomics Laboratory Activities - PlantStress Biotech INCT Sequencing the genotype plant native species from Brazil (pitangueira). Sequencing of transcriptomes associated with the response to drought of native species in Brazil (wild peanuts, pitangueira, Clúsia, and cashew) by large-scale sequencing. Identify and select in silico candidate genes related to drought tolerance of native species (wild peanuts, pitangueira, Clúsia, and cashew). Identify InDels and SNPs in candidate genes of native species associated with the drought response (wild peanuts, pitangueira, and cashew). Validate in vitro the expression profile of candidate genes for drought tolerance obtained in native species of Brazil (wild peanuts, pitangueira, Clúsia, and cashew). Sequencing using the Illumina platform a fraction of small RNAs, and their target mRNAs, and circular RNAs of plants (Arachis, Musa, soybean, pitangueira, and cashew tree) submitted to biotic and/or abiotic stresses. Analyze the sequencing data to check the methylation status of gene promoters in the metabolic pathways of interest. Validate the function of plant genes potentially involved in drought tolerance mechanisms in Arabidopsis, rice or sepia plants through strategies of overexpression or silencing. Organize, maintain and share an in vivo bank of the innovation assets obtained in the project shared by INCT members. Laboratory Description Research efforts in our laboratory are focused on the prospecting and characterization of genes involved in the response of plants to biotic and abiotic stresses. Emphasis: Soybean (Glycine max ), Jackbean (Canavalia ensiformis ) and Solanum nigrum . Approaches: Analysis of gene expression; Gene silencing; Overexpression; Subcellular location of gene products. For genetic transformation, we mainly use particle bombardment, but we are gaining experience in Agrobacterium -mediated transformation. Embryogenic tissues are used as targets for transformation. Research Lines Response of native Neotropical plants and cultivated plants to abiotic stresses: drought and salinity. Identification of genes related to plant adaptation and tolerance to abiotic stresses. Characterization of the interaction network between microRNAs - circRNAs and mRNAs in the regulation of gene expression. Study of the mechanisms and quantification of mRNA editing events in chloroplasts in response to stresses. Our Team Rogerio Margis Team Leader Rogerio Margis completed his doctorate at the Institut de Biologie Moleculaire des Plantes, IBMP of CNRS, at the Université Louis Pasteur of Strasbourg I, France, in 1993. In 2002 he did a post-doctorate related to the processes of RNA interference and production of microRNAs in plants in the CSIRO's Plant Industry in Canberra, Australia. He is currently a CNPq researcher and Full Professor in the Department of Biophysics and Biotechnology Center at the Federal University of Rio Grande do Sul. Full member of the Brazilian Academy of Sciences since 2016. From 1994 to 2003 he was an adjunct professor at UFRJ. At UFRGS, he works as a permanent core researcher and advisor in the graduate programs in Genetics and Molecular Biology (PPGBM) and Cell and Molecular Biology (PPGBCM). Currently participates in research projects related to the themes: RNA interference processes, small RNAs and non-coding RNAs in rice, soybean and native Neotropical species; action of cysteine proteases and their inhibitors; proteins related to abiotic stresses (cold, drought and metals) and oxidative stress (APx and GPx). He works in the areas of Genetics and Biochemistry, with an emphasis on Molecular Biology: regulation of gene expression and molecular markers. In his professional activities, he interacted with more than a hundred national and international collaborators in projects and co-authorship of scientific works. Márcia Pinheiro Margis Graduated in Biological Sciences at the State University of Rio de Janeiro (1983), Master's degree in Biochemistry from the Federal University of Rio de Janeiro (1987) and doctorate in Biologie Moleculaire Des Plantes - Universite de Strasbourg I - France (1993). Full Professor at the Department of Genetics at the Federal University of Rio Grande do Sul. She is a full member of the Brazilian Academy of Sciences. Full member of the CNPq Genetics Advisory Committee (CA-GE) between July 2011 and June 2014. She was a member of the board of the Brazilian Society of Genetics (SBG), having been first treasurer, first secretary, vice president, and president (2016 to 2018). Member of CTNBio between March 2012 and February 2014. Coordinator of the Graduate Programs in Genetics at the Federal University of Rio de Janeiro (2000-2001) and the Federal University of Rio de Grande do Sul (2011 to 2015). Editor of the journal Genetics and Molecular Biology. President of the International Genetics Federation (since September 2018). She has experience in the field of Genetics, with an emphasis on Plant Genetics, working mainly on the following topics: defense responses of plants against abiotic stresses, antioxidant metabolism enzymes and functional plant genomics. Contact Rogerio Margis Universidade Federal do Rio Grande do Sul - Department of Biophysics - Room 206 - LGPP Campus do Vale, Bento Gonçalves Avenue 9500, Building 4342212, Postal Code 91501-910, Porto Alegre, RS, Brazil E-mail: rogerio.margis@ufrgs.br Phone number: +55 51 3008-6234

Explore publications of scientific articles, patents, Thesis and Dissertations developed by INCT PlantStress Biotech. Publications Scientific Papers Patents Dissertations and Thesis

< Back INCT PlantStress Biotech’s Participation in the Crop Genomics for Global Food Security Workshop at PAG 2024 Professor Robert Miller (UnB), a member of the INCT PlantStress Biotech, participated in the 31st Plant and Animal Genome Conference (PAG ) in San Diego, California, USA between 12-17 January 2024 . As an invited speaker at the Crop Genomics for Global Food Security Workshop, he delivered a lecture entitled “Functional Genomics Approaches to Promote Stress Resilience in Musa” with focus on results generated in the INCT project. The fungal pathogen Pseudocercospora musae is responsible for Sigatoka leaf spot disease, which results in considerable economic losses in bananas, especially in the important Cavendish subgroup. A transcriptome analysis of the early-stage immune responses to P. musae was investigated in resistant Musa acuminata Calcutta 4 leaf material. Data on the characterization of the microRNAs in the early-stage immune response was also presented. The PAG conference provides a forum for presentation and discussion on recent developments and future applications for plant and animal genome projects worldwide. The conference is typically attended by scientists from more than 65 countries worldwide.

< Back Research Developing Nematode Control Alternatives Receives Awards at Conference Students Affiliated with INCT Plant Stress Biotech, Developing Nematode Control Alternatives, Win Best Research Awards at the 53rd Brazilian Phytopathology Congress. Nematodes have a significant impact on agriculture by attacking plant roots, disrupting their growth and nutrient uptake. These parasites can cause substantial damage in various crops, including banana and cotton. In the banana crop, for instance, nematodes can weaken plants, displaying symptoms like yellowing, wilting, and even premature death of seedlings. In cotton, these organisms can induce the formation of root galls, impairing water and nutrient absorption, leading to reduced productivity and fiber quality. Cotton Plant Roots Infected by Nematodes. The Marked Areas Are Galls, Structures Formed as a Result of Infection by the Nematode M. incognita. Effectively controlling nematodes remains a constant concern for farmers, as inadequate management can result in significant harvest losses. Techniques like crop rotation, cover cropping, and application of nematicide chemicals are some of the approaches used to mitigate damage caused by these parasites. However, a more sustainable and promising approach is the development of plants resistant to nematodes. Through biotechnology techniques, it's possible to improve the resistance genes into target crops, granting them the ability to better withstand nematode attacks. This approach not only reduces reliance on chemical agents but can also boost crop productivity and quality, thereby contributing to food security and agricultural economies. Best Research in the Undergraduate and Master's Categories Between August 7th and 10th, 2023, the 53rd Brazilian Phytopathology Congress took place in Brasília, Federal District. The event aims to bring together professionals from the fields of education, research, and extension in both the public and private sectors, as well as students from Brazil and abroad, along with experts from various segments of production chains. The Conference objective is to discuss the current status and scientific advancements related to plant diseases and their control methods, fostering knowledge exchange and anticipating new challenges to be overcome. Bringing innovations and promising outcomes to agriculture, Sara Vitorino da Rocha Lemes , a Biotechnology student, and Lucas Santos Bastos , an Agronomist and Master's candidate in the Phytopathology Postgraduate Program, both from the University of Brasília - UnB, were awarded for their research. Sara earned second place in the Undergraduate category, while Lucas secured third place in the Master's category. Sara's work, titled "Development of cotton plants with reduced susceptibility to Meloidogyne incognita through RNA interference". The aim of Sara's work is to develop transgenic cotton plants that produce a specific RNA molecule for nematode control. Therefore, when the parasite feeds on the plant, it also ingests this RNA, hampering its development and reducing the number of galls and eggs in the plant roots. Lucas's master's research aimed to identify and characterize genes associated with resistance to M. incognita and tolerance to water stress, as well as how the interaction between these two stresses occurs in banana plants. Through this study, it becomes possible to enhance our understanding of the mechanisms linked to these stresses and provide data for banana genetic improvement. It was with the work titled "Differential Expression of Genes Associated with Meloidogyne incognita Infection and Water Deficit in Musa acuminata " that he secured third place in his category. These awards showcase the quality and effectiveness of projects affiliated with INCT Plant Stress Biotech, as well as one of INCT's objectives in developing biotechnological tools for the Brazilian agribusiness.

< Back Advances in Research from UFRJ and Embrapa on Coffee Gene that Increases Drought Resistance in Soybeans and Cotton Drought is one of the biggest challenges facing agriculture worldwide, causing significant losses, particularly in key crops such as coffee, soybeans, and cotton. Despite coffee's substantial economic importance, our understanding of how the plant responds to water scarcity at the molecular level is still limited. Research conducted by the Molecular Genetics and Plant Biotechnology Laboratory at UFRJ, led by Professor Márcio Alves-Ferreira, in collaboration with the team from Embrapa Cenargen, coordinated by researcher M. Fatima Grossi de Sa, studied all the "homeobox" (HB) gene family members present in the genome of Coffea arabica . They discovered that three of these genes are activated when the plant experiences osmotic stress (such as drought). Among these, the gene CaHB12 stood out as it is significantly activated in the leaves and roots of coffee plants under moderate and severe drought conditions. To better understand the role of CaHB12 , the scientists introduced this gene into transgenic Arabidopsis plants (a model plant) and observed that these plants exhibited increased tolerance to drought and salt during germination. During the analyses, they noted that while some classic drought response genes showed decreased activity, other pathways, such as those related to heat shock proteins, were activated, which may explain the enhanced resistance of the plants. This study was supported by the INCT PlantStress Biotech and also involved collaborations with the Universidad Nacional del Litoral (Argentina) and Universität Bonn (Germany). Furthermore, the researchers tested the expression of CaHB12 in transgenic soybean and cotton plants, with results indicating increased drought tolerance. The findings of this study, recently published in the journal Environmental and Experimental Botany , suggest that CaHB12 is a positive regulator of the stress response in coffee plants and a promising candidate for biotechnological applications. A diagram provides a graphical summary of the results. Also, check out the dissemination conducted in the FAPERJ newsletter, as well as the original article in the scientific journal. FAPERJ Dissemination https://www.faperj.br/?id=648.7.8 Link to article in EEB https://www.sciencedirect.com/science/article/abs/pii/S0098847224003411

< Back Genetic engineering can have a positive effect on the climate Agriculture accounts for around 25 percent of all greenhouse gas emissions worldwide. A large share of these emissions is due to livestock production and fertilizer use. However, more than one-third of agriculture's emissions is caused by land-use change, especially the conversion of forests and other nature reserves to agricultural land in order to satisfy the rising global demand for food and feed. "Using better technologies to increase crop yields on the land already cultivated could reduce this land-use change and the associated emissions," says study author Prof. Dr. Matin Qaim, Director of the Center for Development Research at the University of Bonn. Certain types of genetically modified crops -- such as GM maize and soybean -- are widely grown in other parts of the world, but hardly in Europe. "The main reasons are public acceptance issues and political hurdles," says Qaim. In the new study, he and his colleagues from the Breakthrough Institute used global agricultural data and estimates of the yield effects of GM crops to model how increased technology adoption in the EU would affect production, land use, and greenhouse gas emissions. The estimates suggest that more widespread use of genetically modified crops in the EU could prevent the release of 33 million tons of CO2 equivalents, which corresponds to 7.5 percent of the EU's total annual greenhouse gas emissions from agriculture. Higher yields in the EU would have a global effect "Most of these positive climate effects are attributable to reduced land-use change," says Dr. Emma Kovak from the Breakthrough Institute, the study's first author. The conclusion of the research team: "The EU imports a lot of maize and soybean from Brazil, where the expansion of agricultural land contributes to tropical deforestation. Higher yields in the EU could reduce some of these imports and thus help preserve the Amazon rainforest." The authors stress that in their analysis they only look at already-existing genetically modified crops. "New genomic breeding technologies are currently being used to develop a wide range of new crop applications that could lead to additional climate change mitigation and adaptation benefits in the future," says Matin Qaim. The agricultural economist is a member of the Transdisciplinary Research Area "Sustainable Futures" and Cluster of Excellence "PhenoRob -- Robotics and Phenotyping for Sustainable Crop Production" at the University of Bonn. Source: Materials provided by University of Bonn . Note: Content may be edited for style and length. Journal Reference : Emma Kovak, Dan Blaustein-Rejto, Matin Qaim. Genetically modified crops support climate change mitigation . Trends in Plant Science , 2022; DOI: 10.1016/j.tplants.2022.01.004

Here you can find all registered patents. Results of works carried out by the INCT PlantStress Biotech network. Publications Patents VASLIN,M. F. S. ; BARRETO-BERGTER, E.; SILVA NETO, M. A. C. ; MONTEBIANCO, C. B. ; BERNARDINO, M. C. ; MATTOS, B. B. ; CARVALHO, S. S. Patent: Privilégio de Inovação. Número do registro: BR-1020170020452 , título: "COMPOSIÇÃO PRAGUICIDA, USO, MÉTODO DE CONTROLE DE PATÓGENOS DE PLANTAS, MÉTODO DE CONTROLE DE PROPAGAÇÃO DE INSETOS E PROCESSO DE OBTENÇÃO DE PROTEÍNAS DE FUNGOS", Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Deposit: January 31, 2017; Patente garanted: September 20, 2022. VASLIN, M.F.S. ; BARRETO-BERGTER, ELIANA; MONTEBIANCO, C. B.; BERNARDINO, M. C.; SANTOS, J. L. J. COMPOSIÇÃO BIOINDUTORA, MÉTODO DE APLICAÇÃO DA COMPOSIÇÃO BIOINDUTORA E SEU USO. 2020, Brasil. Patent: Privilégio de Inovação. Número do registro: BR-10202002635, título: "COMPOSIÇÃO BIOINDUTORA, MÉTODO DE APLICAÇÃO DA COMPOSIÇÃO BIOINDUTORA E SEU USO", Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Deposit: 22/12/2020 HEMERLY, A.S.; Cavalcanti, P.F.; Gong, P.; Nelissen, H.; Inze, D.; Grossi-de-Sa, M.F.; Basso, M.F.; Morgante, C.V.; Lisei-de-Sa, M.E. Method for promoting an increase in plant biomass, productivity, and drought resistance. US 2020/0347399 A1 . Deposit: May 4, 2020. Publication: November 5, 2020. GROSSI-DE-SA, M.F.; Silva, M.C.M.; Fonseca, F.C.A.; Macedo, L.L.P.; Lourenco, I.T.; Albuquerque, E.V.S. Aparato e método de criação de larvas de insetos em laboratório. BR 1020130331120 B1 . Deposit: December 20, 2013. Patent granted: November 24, 2020. GROSSI-DE-SA, M.F.; Oliveira, G.R.; Silva, M.C.M.; Rocha, T.L.; Magalhaes, M.T.Q. Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica. PI 0906128-2 B1 . Deposit: July 24, 2009. Patent granted: March 17, 2020. GROSSI-DE-SA, M.F.; Macedo, L.L.P.; Silva, M.C.M.; Almeida-Garcia, R.; Silva, L.P.; Vila, A. Pesticide. WO 2020/007450 A1 . Deposit: July 7, 2018, Publication: January 9, 2020. GROSSI-DE-SA, M.F.; Macedo, L.L.P.; Pinto, C.E.M.; Leite, A.G.B.; Silva, M.C.M.; Lourenço-Tessutti, I.T.; Morgante, C.V. Método para produzir planta resistente a inseto praga e moléculas de ácido nucleicos utilizado para obtenção de tal planta através de dsRNAs relacionados a ecdise. PI 102020004312-9 . Deposit: March 3, 2020. ROCHA, T.L.; Evaristo, R.G.S.; Grossi-de-Sa, M.F.; Silva, M.C.M.; Polez, V.L.P.; Roessner, U.; Bacic, T. Nematotoxic composition of synergistic effect, use of a nematotoxic composition of synergistic effect. US 9750247 B2, CN 105007725 B . Deposit: December 26, 2013 (US and CH). Patent granted: September 5, 2017 (US) and April 4, 2019 (CH). REMER, R.A.; Margis, R.; Lima, M.C.; Coronha Lima M.; Alves-Ferreira, M. Produto farmacêutico e processo para sua produção. PI 03051978 . Deposit: November 13, 2003 (BR). Publication: November 4, 2019. MACEDO, L.L.P.; Grossi-de-Sa, M.F.; Silva, M.C.M.; Almeida-Garcia, R.; Mendes, R.A.G.; Albuquerque, E.V.S. Increasing the efficiency of supression of gene expression by means of the use of RNA molecules with a stabilized structure. BR 10201700690-4 A2, WO 2018/184083 A1. Deposit: April 4, 2017 (BR) and March 29, 2018 (US). Publication: October 30, 2018 (BR) and November 11, 2018 (US). ALVES-FERREIRA, Marcio; Agropecuária, E. B.; Bencke, M. ; Guimarães-Dias F ; Conforte, A. ; Nepomuceno A ; Grossi-de-As, M. F. . Composições e métodos para modificar a expressão de genes usando promotor de soja induzível por déficit hídrico em plantas. 2018, Brasil. Patente: Privilégio de Inovação. Número do registro: BR1020180037692, título: " Composições e métodos para modificar a expressão de genes usando promotor de soja induzível por déficit hídrico em plantas." Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 26/02/2018 GROSSI-DE-SA, M.F.; Silva, M.C.M.; Del-Sarto, R.P.; Rocha, T.L. Mutantes de inibidores de alfa amilases isolados de Phaseolus vulgaris com propriedades de controlar insetos-praga, composições contendo tais mutantes e métodos de obtenção dos mesmos e de linhagens transgênicas. PI 1102841-6 . Deposit: June 8, 2011. Patent granted: November 26, 2018. VASLIN, M.F.S. ; CORREA, R. L. ; VIDAL, M. S. ; BARROSO, P. A. V. Patente: Privilégio de Inovação. Número do registro: PI-06001513 , título: "TESTE MOLECULAR VOLTADO PARA A IDENTIFICAÇÃO E O DIAGNÓSTICO, IN VITRO, DO VÍRUS RESPONSÁVEL PELA DOENÇA AZUL DO ALGODOEIRO (CLRDV)" , Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Deposit : January 9, 2006; Patent granted : August 15, 2017. GROSSI-DE-SA, M.F.; Coelho, R.R.; Firmino, A.A.P.; Macedo, L.L.P; Silva, M.C.M.; Lourenço-Tessutti, I.T. Método e composições para controle de insetos-praga em plantas por meio do silenciamento de genes da família da quintina sintase e da vitelogenina bem como alternativamente pela expressão do gene de uma toxina Cry. BR 102013032649-6 A2 . Deposit: December 18, 2013. Publication: February 10, 2016. GROSSI-DE-SA, M.F.; Silva, M.C.M.; Macedo, L.L.P.; Firmino, A.A.P.; Coelho, R.R.; Lourenço-Tessuti, I.T. Método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases. BR 102012033539-5 A2 , US 10182571 B2 and WO 2014/100879 A2 . Deposit: December 28, 2012 (BR), December 27, 2013 (US) and June 27, 2013 (WO). Publication: August 18, 2015 (BR), January 22, 2019 (US) and January 3, 2014 (WO). GROSSI-DE-SA, M.F.; Guimaraes, L.M.; Batista, J.A.N.; Viana, A.A.B.; Fragoso, R.R.; Rocha, T.L. Composições e métodos para modificar a expressão de genes usando o promotor do gene da proteína de conjugação à ubiquitina de plantas de algodoeiro. PI 0701230-6 , US 2013/0152226 A1 . Deposit: February 5, 2007 (BR) and February 19, 2013 (US). Patent granted: June 26, 2018 (BR) and June 13, 2013 (US). ALVES-FERREIRA, M.; CRUZ, Fernanda Pinheiro da; Grossi-de-Sá, Maria Fátima ; ROMANO, Eduardo . UTILIZAÇÃO DO GENE HOMEOBOX DE CAFÉ CAHB12 NA PRODUÇÃO DE PLANTAS TRANSGÊNICAS MAIS TOLERANTES AO DÉFICIT HÍDRICO E ESTRESSE SALINO - INPI em 12/11/2009 - Protocolo nº 020100106005. 2010, Brasil. Patente: Privilégio de Inovação. Número do registro: PI020100106005, título: "UTILIZAÇÃO DO GENE HOMEOBOX DE CAFÉ CAHB12 NA PRODUÇÃO DE PLANTAS TRANSGÊNICAS MAIS TOLERANTES AO DÉFICIT HÍDRICO E ESTRESSE SALINO - INPI em 12/11/2009 - Protocolo nº 020100106005" , Instituição de registro: INPI - Instituto Nacional da Propriedade Industrial. Depósito: 12/11/2010; Concessão: 04/09/2019.